Figures
In the Mammospheres are Resistant to Vitamin D Treatment section within the Results Section all instances of nM should appear as μM. One exception in the last sentence; the value (0-100 nM) is correct. There are errors in Figures 1, 7, and 8. Please see the corrected Figures here: Figure 1:
Figure 7: Figure 8: The corrected figure legend of Figure 7 is: Cells were treated with 1,25D (0–0.1 μM) and allowed to proliferate for 4 days under high attachment conditions and cell numbers were counted by trypan blue method (*, p≤0.05; **, p≤0.01). Medium was replaced after every 48 hrs with appropriate concentrations of 1,25D. B, SKBR3 (2×104) cells were plated under mammosphere conditions on a 12-well ultra-low attachment plates in presence of different concentrations of 1,25D (0–0.1 μM) and allowed to grow under mammosphere conditions for 4 or 7 days and sphere diameters were measured. Appropriate concentrations of 1,25D were additionally supplemented in the culture medium after every 48 hours. Left Panel: Micrographs were taken at 100×magnification. Right Panel: Quantitative analysis of average diameter computed from 20 different fields from each treatment group. C, Quantitative real-time PCR analysis of Cyp27 B1 and Cyp24A1 mRNA expression from MCF-7 cells grown under plas or mam conditions after 4 days of plating (**, p≤0.01). D, HPLC analysis of 24,25D3 and 1,25D synthesis in cells grown under plastic or mammos conditions from MCF-7 cells after 4 days of plating (*, p≤0.05; **, p≤0.01). doi:10.1371/journal.pone.0053287.g007 The corrected figure legend of Figure 8 is: Bottom Panel: Immunofluorescence analysis of MKP-1 and pERK1/2 in control and DETA (0.3 mM) treated mammospheres after 24 hrs. B, Top panel: Photomicrographs of HRas cells plated under mammosphere conditions and allowed to grow in medium containing either 1,25D (0.1 μM) and DETA (0.3 mM) alone or in combination for 5 days. Bottom panel; Average diameter of mammospheres computed from 20 different fields from each treatment groups (*, p≤0.05; **, p≤0.01). C, 5×105 HRas cells were allowed to seed on T-25 flasks and treated with 1,25D and DETA either alone or in combination. Total number of cells were counted after 5 days (*, p≤0.05; **, p≤0.01). D, HRas cells were plated under mammospheres conditions and treated with DETA (0.3 mM), or 1,25D (0.1 μM) either alone or in combination for 3 days. Mammospheres were dissociated and 1×105 cells from each treatment groups were injected into nude mice and tumor volumes were analyzed at various time points (1–8 weeks) (*, p≤0.05; **, p≤0.01 compared to the Con group). doi:10.1371/journal.pone.0053287.g008Citation: Pervin S, Hewison M, Braga M, Tran L, Chun R, Karam A, et al. (2013) Correction: Down-Regulation of Vitamin D Receptor in Mammospheres: Implications for Vitamin D Resistance in Breast Cancer and Potential for Combination Therapy. PLoS ONE 8(10): 10.1371/annotation/5326d117-3f31-4e43-a5c4-9e1fb41719e9. https://doi.org/10.1371/annotation/5326d117-3f31-4e43-a5c4-9e1fb41719e9
Published: October 21, 2013
Copyright: © 2013 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Competing interests: No competing interests declared.