Figures
Novel regulatory role of miR-146a identified in Lyme arthritis and macrophage phagocytosis of Borrelia burgdorferi.
Peritoneal macrophages from miR-146a-/- mice were incubated with GFP-labeled B. burgdorferi and phagocytic uptake monitored by confocal microscopy. Apparent in the overlay are cell nuclei (gray), lysosomes (red), cell membranes (blue), and B. burgdorferi (green). B. burgdorferi are identified as bright GFP puncta associated with intracellular lysosomes and as spiral shaped bacteria on the cell surface. Uptake of B. burgdorferi was greater in macrophages lacking miR-146a than wild type macrophages, consistent with the reduced bacterial presence and increased Lyme arthritis severity in joint tissue of infected miR-146a-/- mice. See Lochhead et al.
Image Credit: Robert B Lochhead, Janis J Weis
Citation: (2014) PLoS Pathogens Issue Image | Vol. 10(6) June 2014. PLoS Pathog 10(6): ev10.i06. https://doi.org/10.1371/image.ppat.v10.i06
Published: June 26, 2014
Copyright: © 2014 Lochhead et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Peritoneal macrophages from miR-146a-/- mice were incubated with GFP-labeled B. burgdorferi and phagocytic uptake monitored by confocal microscopy. Apparent in the overlay are cell nuclei (gray), lysosomes (red), cell membranes (blue), and B. burgdorferi (green). B. burgdorferi are identified as bright GFP puncta associated with intracellular lysosomes and as spiral shaped bacteria on the cell surface. Uptake of B. burgdorferi was greater in macrophages lacking miR-146a than wild type macrophages, consistent with the reduced bacterial presence and increased Lyme arthritis severity in joint tissue of infected miR-146a-/- mice. See Lochhead et al.
Image Credit: Robert B Lochhead, Janis J Weis