Figures
Multicolored syncytia induced by morbilliviruses isolated from ferret white blood cells
Vero cells expressing canine SLAM were co-cultured with white blood cells of ferrets. The animals had been inoculated via different routes with recombinant canine distemper viruses expressing the fluorescent proteins Venus, dTom or TagBFP. Systemic virus dissemination in vivo resulted in circulation of single, double- and triple-infected lymphocytes. Morbillivirus isolation in vitro induced formation of multinucleated syncytia of which the cytoplasm was flooded with different combinations of fluorescent reporter proteins. This virus competition model will facilitate identification of factors associated with viral fitness and transmission. Image taken by confocal laser scanning microscopy. de Swart et al.
Image Credit: Rory de Vries, Paul Duprex and Rik de Swart
Citation: (2017) PLoS Pathogens Issue Image | Vol. 13(5) May 2017. PLoS Pathog 13(5): ev13.i05. https://doi.org/10.1371/image.ppat.v13.i05
Published: May 31, 2017
Copyright: © 2017 de Vries, Duprex, and de Swart. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Vero cells expressing canine SLAM were co-cultured with white blood cells of ferrets. The animals had been inoculated via different routes with recombinant canine distemper viruses expressing the fluorescent proteins Venus, dTom or TagBFP. Systemic virus dissemination in vivo resulted in circulation of single, double- and triple-infected lymphocytes. Morbillivirus isolation in vitro induced formation of multinucleated syncytia of which the cytoplasm was flooded with different combinations of fluorescent reporter proteins. This virus competition model will facilitate identification of factors associated with viral fitness and transmission. Image taken by confocal laser scanning microscopy. de Swart et al.
Image Credit: Rory de Vries, Paul Duprex and Rik de Swart