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How Do Filamentous Pathogens Deliver Effector Proteins into Plant Cells?

Abstract

Fungal and oomycete plant parasites are among the most devastating pathogens of food crops. These microbes secrete effector proteins inside plant cells to manipulate host processes and facilitate colonization. How these effectors reach the host cytoplasm remains an unclear and debated area of plant research. In this article, we examine recent conflicting findings that have generated discussion in the field. We also highlight promising approaches based on studies of both parasite and host during infection. Ultimately, this knowledge may inform future broad spectrum strategies for protecting crops from such pathogens.

Introduction

Fungi and oomycetes are eukaryotic filamentous microbes, some of which are devastating plant pathogens that affect important food crops. For instance the oomycete potato blight pathogen Phytophthora infestans triggered the Irish famine during the 19th century and remains the most important threat to potato production, whereas fungi such as the ascomycete rice blast pathogen Magnaporthe oryzae and the basidiomycete wheat stem rust pathogen Puccinia graminis f. sp. tritici continuously threaten global food security [1],[2]. During infection, these parasites engage in complete or partial biotrophic interactions, meaning that they develop feeding relationships with the living cells of their hosts by intimately associating with plant tissues. These microbes differentiate specialized parasitic structures within infected tissues, such as hyphae, which explore the extracellular space (apoplast), or invasive hyphae and haustoria, which penetrate host cell cavities and invaginate the host's plasma membrane (Figure 1) [3],[4]. Historically, hyphae and haustoria have been described as feeding structures that serve the nutrition of the parasites. But more recently these structures have emerged as sites of secretion and translocation into host cells of a class of pathogen virulence proteins known as effectors (Figure 1) [5],[6].

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Figure 1. Fungal and oomycete structures for effector secretion.

Left panel. Oomycete and fungal plant parasites differentiate infection structures such as extracellular hyphae, as well as invasive hyphae and haustoria that penetrate the host cell cavity and invaginate the plasma membrane. Haustoria (a) and hyphae (b) secrete effectors that are translocated into host cell cytoplasm by unknown mechanisms. Right panel. Effectors secreted from haustoria (a) and hyphae (b) cross different biological interfaces (extra-haustorial matrix [EHMx]/extra-haustorial membrane [EHM] for effectors secreted from haustoria, and apoplast/plant cell wall/plant plasma membrane for effectors secreted from hyphae).

https://doi.org/10.1371/journal.pbio.1001801.g001

Effectors manipulate plant processes to the advantage of the parasite, promoting host infection and colonization, yet they may also activate plant immune receptors on resistant host genotypes [7]. During the past decade, it has become apparent that numerous fungal and oomycete effectors operate inside the host cell cytoplasm [8][11], extending to these pathogens a concept first put forward for plant pathogenic bacteria [12]. Nevertheless, the mechanisms by which effector proteins traffic to the plant cell cytoplasm remain poorly understood in contrast to the well-studied bacterial secretion systems. Solving the enigma of how filamentous pathogens deliver their effectors to the inside of plant cells is a fundamental question in plant pathology. Moreover, the prevention of effector secretion or internalization into host cells is likely to interfere with parasitic growth, thus representing a potential crop protection strategy for use in agriculture. Also, effectors target different host subcellular compartments and mediate a variety of biochemical modifications, thus representing valuable molecular tools for fundamental and applied plant biology studies [7],[13].

Filamentous pathogen effector proteins that translocate into plant cells are highly diverse in sequence and structure and have most likely evolved a variety of mechanisms to traffic to the host cytoplasm. However, a common theme is that host-targeting relies on N-terminal translocation domains that are located after a general secretory signal peptide (Figure 2). In the oomycetes, host-targeting domains contain overrepresented motifs, such as the RXLR, LFLAK, and CHXC amino acid sequences, which define many predicted effector repertoires in different species [14]. In one early study, Whisson and colleagues (2007) showed that the N-terminus of the AVR3a effector from P. infestans is required for translocation into potato cells, a finding that supported the view that the RXLR domain functions as a leader sequence that mediates host cell targeting [5].

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Figure 2. N-terminal effector domains proposed to mediate host-cell entry.

Effectors from fungal (left) and oomycete (right) pathogens. Divergent oomycete and fungal effectors carry a general secretion signal peptide followed by non-conserved N-terminal regions called “uptake” or “targeting/translocation” domains that have been proposed to mediate host-cell entry. In oomycetes, small conserved amino acids motifs (e.g., RXLR, CHXC, or LFLAK) have been identified within these regions, which help to define effector families with many members.

https://doi.org/10.1371/journal.pbio.1001801.g002

Identification of motifs involved in cell entry is not as advanced for fungal effectors as it is for oomycetes. Large families of candidate effectors have been identified from fungal genomes, largely on the basis of predicted N-terminal signal peptides, small size, and lack of similarity to other proteins [15],[16]. Additionally, sequences that mediate host-cell translocation have been detected within host-specific toxins of necrotrophic fungi. One well-studied example is the C-terminal RGD motif of ToxA from Pyrenophora tritici-repentis, which is required for entry into host plant cells [17]. Also, domains in the N-termini of the flax rust fungus Melampsora lini effectors AvrM and AvrL567 mediate uptake into plant cells, although whether these sequences determine entry into plant cells or other processes, such as escape from plant endosomes following endocytosis, is still unclear (see below) [6],[18]. However, a consensus cell entry motif for fungal proteins, analogous to the common RXLR in oomycetes, has not been defined.

Current Models and Controversies

A number of studies aimed at elucidating the function of N-terminal host-targeting domains of filamentous plant pathogen effectors have been published and are summarized in Table 1. Monitoring effector trafficking from the parasite to the host cell is technically challenging. Indeed, many filamentous plant pathogens are not amenable to genetic manipulation, and the direct visualization of effector proteins during infection has proven to be elusive. In addition, effectors traffic across dynamic interfaces, such as haustoria, that can only form inside host tissue. As a consequence, the results and models generated to date are mostly based on proxy experiments conducted independently of the pathogen. They essentially tackle the question of “how effectors cross the host plasma membrane” (summarized in [19]), leading to a model that involves “autonomous” or “pathogen-independent” host cell entry [6],[20][22]. Kale and colleagues (2010) also proposed a mechanistic model for this phenomenon. The RXLR motif in oomycetes or degenerate RXLR-like motifs in fungi define cell entry domains and bind extracellular phosphatidylinositol-3-phosphate (PI3P) to mediate effector endocytosis into host cells [22],[23]. However, the experimental findings that underpin this and related models have proven controversial with several studies alternatively supporting or challenging the reproducibility of the assays and the robustness of the conclusions (see Table 1 for details).

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Table 1. List of conflicting studies on filamentous pathogen effector translocation inside plant cells.

https://doi.org/10.1371/journal.pbio.1001801.t001

First, the occurrence of RXLR-like motifs in fungal effectors [22],[23] that are functionally and structurally related to oomycete RXLR motifs is questionable [18]. The RXLR consensus, associated sequence motifs, and their position near N-termini helped to define the RXLR effector superfamily, which includes hundreds of divergent members in the Phytophthora species, most of which (87%) carry the RXLR sequence [14],[24]. Although variants have been detected, notably QLLR and GKLR in some downy mildew pathogens, the RXLR motif is highly conserved in Phytophthora effectors even though these proteins are rapidly evolving and can display high levels of amino acid polymorphism [14],[24],[25]. This indicates that the RXLR motif is mostly under purifying selection, meaning that variants that have arisen have been mostly eliminated by natural selection. Nonetheless, Kale and colleagues (2010) used extensive mutagenesis studies of this sequence combined with cell re-entry and uptake assays (see next paragraph) to show that the motif is highly plastic and that some fungal effectors carry N-terminal RXLR-like motifs, which are highly degenerate as [RHK]X[LMIFYW] [22]. By using similar assays, some authors reported the existence of functional RXLR-like motifs in various fungal effectors, whereas others did not (Table 1). Interestingly, structural investigations of the oomycete effectors Avr3a4 and Avh5 revealed that RXLR domains are intrinsically disordered [8],[26]. In contrast, RXLR-like motifs of the fungal effectors AvrL567 and AvrM are embedded in well-defined structures [18],[27]. Hence, based on the few structures currently available, amino acids similarities within the effector primary sequences are not matched by their structural properties.

Second, the two main assays used to demonstrate pathogen-independent effector entry into host cells are under debate. One such method, the “cell re-entry assay,” is based on the heterologous expression of a full-length effector protein, including its secretion signal peptide, in a plant cell. The expressed effector, or effector-fluorescent protein fusion, is secreted into the extracellular space (apoplast), and its re-entry into the plant cell is tracked [20]. This method has been used to report autonomous cell entry of several fungal and oomycete effectors and to identify the uptake domains required for entry [6],[21],[22]. Nevertheless, this assay cannot unambiguously demonstrate that effectors are indeed secreted into the apoplast prior to cell re-internalisation [28] and it is therefore not possible to exclude that effectors escape the secretory pathway and end up inside the host cytoplasm without crossing the plasma membrane. This limitation of the cell re-entry assay prompted some authors to complement their experiments with a second assay—the “uptake assay”—in which purified recombinant effectors fused to a fluorescent tag are applied to plant tissue, often roots, and their entry followed by microscopy [21][23]. Recently, the robustness and specificity of this method have been debated (Table 1) [29],[30]. Wawra and collaborators (2013) proposed that the process of protein internalization by root cells is non-specific and thus could not inform cell entry mechanisms [29]. Their point was supported by the observation that fluorescent proteins alone are taken up by plant cells at a rate comparable to effector-fluorescent protein fusions. In response, Tyler and colleagues (2013) state that quantitative differences could still be observed, and reported increased entry into cells when fluorescent proteins are fused with effectors or effector uptake domains [30].

Finally, there have been conflicting reports as to whether oomycete RXLR domains can bind phospholipids to mediate cell entry (Table 1). Bhattacharjee and colleagues (2012) confirmed that the RXLR domain of P. infestans effector NUK10 binds PI3P but proposed that this binding takes place inside the pathogen [31]. Sun and colleagues (2013) further investigated the P. sojae effector Avh5 revealing stronger PI3P binding in the C-terminal domain relative to the RXLR domain, but implicating both regions in cell entry [26]. Other studies showed that amino acids residues in the C-terminal half of some oomycete RXLR effectors, rather than in the N-terminus, bind phospholipids and may have a function unrelated to cell entry (Table 1) [8]. Consistent with this idea, some have proposed that phospholipid binding stabilises effectors [26], possibly inside host cells, rather than onto the external surface of the host plasma membrane [8]. Wawra and collaborators (2012) also showed that phospholipid binding of the RXLR effector Avr3a can occur with denatured proteins, and thus questioning the physiological relevance of phospholipid binding [32].

In conclusion, many aspects of the mechanisms by which fungal and oomycete effectors enter into plant cells remain unresolved. There is therefore an urgent need to complement evidence from proxy assays with novel experimental approaches to shed new light on this process.

Towards a Solution: Integrated Pathogen-Host Studies

Our basic understanding of effector trafficking has been hampered by our inability to follow effector secretion and translocation during infection. During translocation, effectors cross several biological interfaces that can be modified during the interaction, as well as new infection-specific compartments (Figure 3) [33]. For instance, haustoria are enveloped by a newly formed membrane called the extrahaustorial membrane (EHM), which differs in protein composition to the plant plasma membrane [34]. These infection-specific biological interfaces are probably mediated by both parasite- and plant-derived factors that need to be taken into account, as they could well influence, if not mediate, effector translocation.

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Figure 3. Integrated process of effector translocation.

Effectors (blue) follow secretion routes (arrows) within a pathogen (orange), are secreted into host-parasite interfaces (grey), cross a membrane surrounding the host cell (green), and finally enter the host cell cytoplasm. Each translocation step is likely to be influenced by host- and parasite-derived mechanisms that need to be considered when studying effector trafficking.

https://doi.org/10.1371/journal.pbio.1001801.g003

The major challenge for the community is methodological. We therefore need to develop genetic, biochemical, and cell biological methods to manipulate, tag, detect, and observe effectors during infection. A growing number of oomycete and fungal plant pathogens are now genetically transformable, thus enabling more pathogen-centered studies. Examples of the value of pathogen-focused studies come from the interaction between M. oryzae and the host plant rice [33] or Ustilago maydis and maize [10]. These pathogens produce invasive hyphae that invaginate the host cell plasma membrane. The use of M. oryzae strains that express fluorescently tagged effectors combined with live-cell imaging has revealed that a highly localized structure, called the biotrophic interfacial complex (BIC), accumulates effectors secreted from the invasive hyphae prior to translocation into the host cell [11],[35]. Such experimental systems should allow further insight into effector trafficking by, for example, addressing the contribution of specific residues within effectors, the influence of infection conditions on effector translocation, and the degree to which plant-derived molecules affect translocation (Figure 3).

The presence of predicted signal peptides in effector proteins has led to the assumption that effectors follow the typical eukaryotic endoplasmic reticulum (ER)/Golgi secretory pathway. As a consequence, the secretion routes followed by effectors inside the pathogen, prior to their secretion and translocation into host cells, have been poorly studied but could turn out to be important as in the case of apicomplexan parasites [31]. For instance, Yi and colleagues (2009) reported that the ER-resident chaperone LHS1 of M. oryzae interferes with effector accumulation at the BIC, and possibly effector secretion [36]. Interestingly, a recent paper combined cell biology with pharmacological approaches to identify two distinct effector secretion pathways in M. oryzae [37]. Whereas apoplastic effectors follow the conventional ER/Golgi secretory pathway, host-translocated effectors appear to follow an alternative secretion route. The extent to which effectors from other pathogens are sorted into distinct secretory pathways remains unknown.

Biochemical approaches need to be explored too. For instance, immunoprecipitation of tagged effectors during the course of infection could reveal the formation of effector-associated protein complexes during the different steps of secretion and translocation. Mass spectrometry associated with biochemical cell fractionation should also enable high throughput subcellular localisation of proteins. Such methods could be applied to colonized tissues, and would assign effectors to different plant subcellular compartments, thus providing evidence of secretion and other valuable information for further characterisation [10].

Finally, although the use of proxy assays alone is unlikely to reveal the full process of effector trafficking, they remain the only alternative in several pathogen systems, and could still provide valuable clues. Some of the established methods, such as the plant cell re-entry assays, need to be better understood. For instance stable transgenic plants expressing fluorescently tagged effector proteins driven by cell-specific promoters should be assayed. The precise fate of heterologously expressed effector proteins also needs to be determined using cell biological and biochemical methods, and the use of multiple tagged proteins tested. Moreover, reagents should be shared between labs and there should be less reliance on transient expression assays.

In conclusion, the targeting of pathogen effectors to the cytoplasm of their plant hosts is a complex process that involves numerous steps (Figure 3). Studies to date have provided some valuable information on effector trafficking in many systems, but new methods are needed to uncover a more comprehensive picture of this process—ideally integrated experimental systems that will allow the detection and visualization of effectors as they traffic from the parasite to the host cell.

Acknowledgments

We thank Tolga Bozkurt, Yasin Dagdas, Kentaro Yoshida, John Rathjen, Kai P. Rinha, and Silke Robatzek for their suggestions. We are also grateful to those who contributed to a broadly diffused community email discussion, as well as those who commented on the Twitter and Scoop.it social networks, for helping to sharpen our thoughts. We apologize for limited literature coverage owing to space limitation.

Author Contributions

The author(s) have made the following declarations about their contributions: Analyzed the data: SK BP. Wrote the paper: SK BP.

References

  1. 1. Yoshida K, Schuenemann VJ, Cano LM, Pais M, Mishra B, et al. (2013) The rise and fall of the Phytophthora infestans lineage that triggered the Irish potato famine. eLife 2: e00731.
  2. 2. Fisher MC, Henk DA, Briggs CJ, Brownstein JS, Madoff LC, et al. (2012) Emerging fungal threats to animal, plant and ecosystem health. Nature 484: 186–194.
  3. 3. Rafiqi M, Ellis JG, Ludowici VA, Hardham AR, Dodds PN (2012) Challenges and progress towards understanding the role of effectors in plant-fungal interactions. Curr Opin Plant Biol 15: 477–482.
  4. 4. Bozkurt TO, Schornack S, Banfield MJ, Kamoun S (2012) Oomycetes, effectors, and all that jazz. Curr Opin Plant Biol 15: 483–492.
  5. 5. Whisson SC, Boevink PC, Moleleki L, Avrova AO, Morales JG, et al. (2007) A translocation signal for delivery of oomycete effector proteins into host plant cells. Nature 450: 115–118.
  6. 6. Rafiqi M, Gan PH, Ravensdale M, Lawrence GJ, Ellis JG, et al. (2010) Internalization of flax rust avirulence proteins into flax and tobacco cells can occur in the absence of the pathogen. Plant Cell 22: 2017–2032.
  7. 7. Win J, Chaparro-Garcia A, Belhaj K, Saunders DG, Yoshida K, et al. (2012) Effector biology of plant-associated organisms: concepts and perspectives. Cold Spring Harb Symp Quant Biol 77: 235–47.
  8. 8. Yaeno T, Li H, Chaparro-Garcia A, Schornack S, Koshiba S, et al. (2011) Phosphatidylinositol monophosphate-binding interface in the oomycete RXLR effector AVR3a is required for its stability in host cells to modulate plant immunity. Proc Natl Acad Sci U S A 108: 14682–14687.
  9. 9. Dong S, Yin W, Kong G, Yang X, Qutob D, et al. (2011) Phytophthora sojae avirulence effector Avr3b is a secreted NADH and ADP-ribose pyrophosphorylase that modulates plant immunity. PLoS Pathog 7: e1002353.
  10. 10. Djamei A, Schipper K, Rabe F, Gosch A, Vincon V, et al. (2011) Metabolic priming by a secreted fungal effector. Nature 478: 395–398.
  11. 11. Park CH, Chen S, Shirsekar G, Zhou B, Khang CH, et al. (2012) The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in rice. Plant Cell 24: 4748–4762.
  12. 12. Gopalan S, Bauer DW, Alfano JR, Loniello AO, He SY, et al. (1996) Expression of the Pseudomonas syringae avirulence protein AvrB in plant cells alleviates its dependence on the hypersensitive response and pathogenicity (Hrp) secretion system in eliciting genotype-specific hypersensitive cell death. Plant Cell 8: 1095–1105.
  13. 13. Bozkurt TO, Schornack S, Win J, Shindo T, Ilyas M, et al. (2011) Phytophthora infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface. Proc Natl Acad Sci U S A 108: 20832–20837.
  14. 14. Jiang RH, Tripathy S, Govers F, Tyler BM (2008) RXLR effector reservoir in two Phytophthora species is dominated by a single rapidly evolving superfamily with more than 700 members. Proc Natl Acad Sci U S A 105: 4874–4879.
  15. 15. Spanu PD, Abbott JC, Amselem J, Burgis TA, Soanes DM, et al. (2010) Genome expansion and gene loss in powdery mildew fungi reveal tradeoffs in extreme parasitism. Science 330: 1543–1546.
  16. 16. Duplessis S, Cuomo CA, Lin Y-C, Aerts A, Tisserant E, et al. (2011) Obligate biotrophy features unraveled by the genomic analysis of rust fungi. Proc Natl Acad Sci U S A 108: 9166–9171.
  17. 17. Manning VA, Hamilton SM, Karplus PA, Ciuffetti LM (2008) The Arg-Gly-Asp-containing, solvent-exposed loop of Ptr ToxA is required for internalization. Mol Plant Microbe Interact 21: 315–325.
  18. 18. Ve T, Willias SJ, Catanzariti A-M, Rafiqi M, Rahman M, et al. (2013) Structures of the flax-rust effector AvrM reveal insights into the molecular basis of plant-cell entry and effector-triggered immunity. Proc Natl Acad Sci U S A 110: 17594–17599.
  19. 19. Kale SD, Tyler BM (2011) Entry of oomycete and fungal effectors into plant and animal host cells. Cell Microbiol 13: 1839–1848.
  20. 20. Catanzariti A-M, Dodds PN, Lawrence GJ, Ayliffe MA, Ellis JG (2006) Haustorially expressed secreted proteins from flax rust are highly enriched for avirulence elicitors. Plant Cell 18: 243–256.
  21. 21. Dou D, Kale SD, Wang X, Jiang RH, Bruce NA, et al. (2008) RXLR-mediated entry of Phytophthora sojae effector Avr1b into soybean cells does not require pathogen-encoded machinery. Plant Cell 20: 1930–1947.
  22. 22. Kale SD, Gu B, Capelluto DG, Dou D, Feldman E, et al. (2010) External lipid PI3P mediates entry of eukaryotic pathogen effectors into plant and animal host cells. Cell 142: 284–295.
  23. 23. Plett JM, Kemppainen M, Kale SD, Kohler A, Legué V, et al. (2011) A secreted effector protein of Laccaria bicolor is required for symbiosis development. Curr Biol 21: 1197–1203.
  24. 24. Haas BJ, Kamoun S, Zody MC, Jiang RHY, Handsaker RE, et al. (2009) Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature 461: 393–398.
  25. 25. Win J, Morgan W, Bos J, Krasileva KV, Cano LM, et al. (2007) Adaptive evolution has targeted the C-terminal domain of the RXLR effectors of plant pathogenic oomycetes. Plant Cell 19: 2349–2369.
  26. 26. Sun F, Kale SD, Azurmendi HF, Li D, Tyler BM, et al. (2013) Structural basis for interactions of the Phytophthora sojae RxLR effector Avh5 with phosphatidylinositol 3-phosphate and for host cell entry. Mol Plant Microbe Interact 26: 330–344.
  27. 27. Wang CI, Guncar G, Forwood JK, Teh T, Catanzariti A-M, et al. (2007) Crystal structures of flax rust avirulence proteins AvrL567-A and -D reveal details of the structural basis for flax disease resistance specificity. Plant Cell 19: 2898–2912.
  28. 28. Oh S-K, Young C, Lee M, Oliva R, Bozkurt TO, et al. (2009) In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes, including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2. Plant Cell 21: 2928–2947.
  29. 29. Wawra S, Djamei A, Albert I, Nürnberger T, Kahmann R, et al. (2013) In vitro translocation experiments with RxLR-reporter fusion proteins of Avr1b from Phytophthora sojae and AVR3a from Phytophthora infestans fail to demonstrate specific autonomous uptake in plant and animal cells. Mol Plant Microbe Interact 26: 528–536.
  30. 30. Tyler BM, Kale SD, Wang Q, Tao K, Clark HR, et al. (2013) Microbe-independent entry of oomycete RxLR effectors and fungal RxLR-like effectors into plant and animal cells is specific and reproducible. Mol Plant Microbe Interact 26: 611–616.
  31. 31. Bhattacharjee S, Stahelin RV, Speicher KD, Speicher DW, Haldar K (2012) Endoplasmic reticulum PI(3)P lipid binding targets malaria proteins to the host cell. Cell 148: 201–212.
  32. 32. Wawra S, Agacan M, Boddey JA, Davidson I, Gachon CM, et al. (2012) Avirulence protein 3a (AVR3a) from the potato pathogen Phytophthora infestans forms homodimers through its predicted translocation region and does not specifically bind phospholipids. J Biol Chem 287: 38101–38109.
  33. 33. Yi M, Valent B (2013) Communication between filamentous pathogens and plants at the biotrophic interface. Annu Rev Phytopathol 51: 567–611.
  34. 34. Lu Y-J, Schornack S, Spallek T, Geldner N, Chory J, et al. (2012) Patterns of plant subcellular responses to successful oomycete infections reveal differences in host cell reprogramming and endocytic trafficking. Cell Microbiol 14: 682–697.
  35. 35. Khang CH, Berruyer R, Giraldo MC, Kankanala P, Park SY, et al. (2010) Translocation of Magnaporthe oryzae effectors into rice cells and their subsequent cell-to-cell movement. Plant Cell 22: 1388–1403.
  36. 36. Yi M, Chi MH, Khang CH, Park SY, Kang S, et al. (2009) The ER chaperone LHS1 is involved in asexual development and rice infection by the blast fungus Magnaporthe oryzae. Plant Cell 21: 681–695.
  37. 37. Giraldo MC, Dagdas YF, Gupta YK, Mentlak TA, Yi M, et al. (2013) Two distinct secretion systems facilitate tissue invasion by the rice blast fungus Magnaporthe oryzae. Nat Commun 4: 1996.
  38. 38. Bos JIB, Kanneganti T-D, Young C, Cakir C, Huitema E, et al. (2006) The C-terminal half of Phytophthora infestans RXLR effector AVR3a is sufficient to trigger R3a-mediated hypersensitivity and suppress INF1-induced cell death in Nicotiana benthamiana. Plant J 48: 165–176.
  39. 39. Gan PHP, Rafiqi M, Ellis JG, Jones DA, Hardham AR, et al. (2010) Lipid binding activities of flax rust AvrM and AvrL567 effectors. Plant Signal Behav 5: 1–4.
  40. 40. Gu B, Kale SD, Wang Q, Wang D, Pan Q, et al. (2011) Rust secreted protein Ps87 is conserved in diverse fungal pathogens and contains a RXLR-like motif sufficient for translocation into plant cells. PLOS ONE 6: e27217.
  41. 41. Ribot C, Césari S, Abidi I, Chalvon V, Bournaud C, et al. (2013) The Magnaporthe oryzae effector AVR1-CO39 is translocated into rice cells independently of a fungal-derived machinery. Plant J 74: 1–12.
  42. 42. Yaeno T, Shirasu K (2013) The RXLR motif of oomycete effectors is not a sufficient element for binding to phosphatidylinositol monophosphates. Plant Signal Behav 8: e23865.
  43. 43. Na R, Yu D, Qutob D, Zhao J, Gijzen M (2013) Deletion of the Phytophthora sojae Avirulence Gene Avr1d Causes Gain of Virulence on Rps1d. Mol Plant Microbe Interact 26: 969–976.