Figures
An influenza-specific CD8+ T cell peptide encoded within the nucleoprotein (NP) was incorrectly referred to as NP366-372 throughout the article. The correct designation should be NP366-374. The peptide sequence ASNENMETM stated throughout the ‘Methods’ section should also be attributed to NP366-374.
The authors have provided corrected versions of Fig 3, Fig 4, Fig 5, Fig 8, S10 Fig and S11 Fig.
C57.BL/6 and Adamts5-/- mice were intranasally (i.n.) infected (104 pfu/mouse) with X31 (H3N2) influenza virus. Spleens were removed at day 7 and day 10 p.i., and CD8+ T cell responses determined. Total CD4+ and CD8+ T cells numbers were calculated at days (A) 7 and (B) 10 p.i. Influenza-specific DbNP366-374 CD8+ and DbPA224-233 CD8+ tetramer-positive T cell numbers were measured at days (C) 7 and (D) 10 p.i. Functional influenza-specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell activity was determined by ICS, and total IFNγ+ T cells enumerated at days (E) 7 and (F) 10 after infection. WT denotes C57.BL/6. The results are expressed as means ± SD, and statistical significance (relative to C57.BL/6) was determined by Student’s t test (*p ≤ 0.05, **p ≤ 0.01, n = 5 representing three experiments). Underlying data are provided in S1 Data.
C57.BL/6 and Adamts5-/- mice were infected i.n. with 104pfu X31 (H3N2) influenza virus. Lungs were removed at days 7 and 10 p.i., and CD8+ T cell immunity characterised. Total CD4+ and CD8+ T cell numbers are shown at days (A) 7 and (B) 10 p.i. Influenza-specific tetramer+ DbNP366-374+ CD8+ and DbPA224-233+ CD8+ T cell responses were enumerated at days (C) 7 and (D) 10 p.i. CD8+ T cell functionality was assessed by ICS and DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses enumerated at days (E) 7 and (F) 10 p.i. Wildtype denotes C57.BL/6 mice. The results are expressed as means ± SD, and statistical significance (relative to C57.BL/6) was determined by Student’s t test (* = p ≤ 0.05, ** = p ≤ 0.01, n = 5 representing three experiments). Underlying data are provided in S1 Data.
C57.BL/6 and Adamts5-/- mice were infected i.n. with 104 pfu/mouse X31 (H3N2) influenza virus. MLNs were removed, pooled, and processed at days 7 and 10 p.i., and single-cell suspensions analysed for influenza-specific immunity. Total CD4+ and CD8+ T cell numbers were determined at days (A) 7 and (B) 10 p.i. Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cells were enumerated at days (C) 7 and (D) 10 p.i. CD8+ T cell functionality was measured using ICS. Influenza-specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses were characterised at days (E) 7 and (F) 10 p.i. Results are expressed as total pooled means from five mice repeated three times. Wildtype denotes C57.BL/6 mice. Underlying data are provided in S1 Data.
Spleen and MLNs were removed from C57.BL/6, Adamts5-/-Vcan+/+, and Adamts5-/-Vcan+/hdf mice and processed at day 10 p.i., and single cell suspensions were analysed for influenza-specific immunity. (A) Total CD8+ T cell numbers were determined at day 10 p.i. in the spleen. (B) Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cells in the spleen were enumerated at day 10 p.i. CD8+ T cell functionality was measured using ICS. (C) Influenza specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses were characterised in the spleen at days 10 p.i. (D) Total CD8+ T cell numbers were assessed at day 10 p.i. in the pooled MLN. (E) Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cells in the pooled MLN were enumerated at day 10 p.i. CD8+ T cell functionality was measured using ICS. (F) Influenza-specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses were characterised in the pooled MLN at day 10 p.i. The results are expressed as means ± SD (spleen data) or as pooled means (MLN data), and statistical significance (relative to C57.BL/6 mice) was determined by one-way ANOVA (*p ≤ 0.05, ***p ≤ 0.005 relative to C57.BL/6, n = 5 representing three individual experiments). WT denotes C57.BL/6 mice and ts5-/- denotes Adamts5-/-. (G) Our model for ADAMTS5 enzyme activity and T cell migration proposes that versican can inhibit T cell effector function by acting as a physical block. Cleavage of versican by ADAMTS5 removes the ECM blockade, allowing migration (top panel). Moreover, versican accumulation in the absence of ADAMTS enzyme activity results in T cell clustering (bottom panel). Underlying data are provided in S1 Data.
The relevant figure legends have been amended to reflect this change and are presented below.
Supporting information
S10 Fig. Influenza virus infection of Vcan+/hdf mice.
Lung tissue and MLNs were removed from influenza virus infection C57.BL/6 and Vcan+/hdf mice and processed to generate single cell suspensions at day 10 p.i. for analysis of influenza-specific immunity. (A) Total CD8+ T cell numbers were determined at day 10 p.i. in the lung. (B) Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cells in the lung were enumerated at day 10 p.i. CD8+ T cell functionality was measured using ICS. (C) Influenza specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses were characterised in the lung at day 10 p.i. (D) Total CD8+ T cell numbers were determined at day 10 p.i. from pooled MLN samples. (E) Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cells in pooled MLN were enumerated at day 10 p.i. (F) CD8+ T cell functionality was measured using ICS to assess influenza-specific DbNP366-374+IFNγ+CD8+ and DbPA224-233+IFNγ+CD8+ T cell responses at day 10 p.i. The results are expressed as means ± SD or as pooled means (MLN data) and statistical significance (relative to C57.BL/6 mice) determined by a Student’s t test (*p ≤ 0.05, ***p ≤ 0.005 relative to C57.BL/6, n = 5 representing three individual experiments). WT denotes C57.BL/6 mice. Underlying data are provided in S2 Data.
https://doi.org/10.1371/journal.pbio.3000558.s001
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S11 Fig. Influenza infection of Adamts5-/- and WT littermate controls.
Adamts5-/- and WT mice were infected i.n with X31 (H3N2) influenza virus and spleens, lungs, and MLNs removed from C57.BL/6 and Adamts5-/- mice days 7 p.i. Single cell suspensions were then analysed for influenza-specific immunity. (A) Weight loss was calculated over the time course of infection. Total CD4+ and CD8+ T cells were enumerated in the (B) spleen, (C) lung, and (D) MLN. Influenza-specific DbNP366-374+ CD8+ and DbPA224-233+ CD8+ tetramer positive T cell numbers were also characterised in the (B) spleen, (C) lung, and (D) MLN. Lung and spleen results are expressed as means ± SD or as pooled means (MLN data), and statistical significance (relative to C57.BL/6 mice) was determined by a Student’s t test (*p ≤ 0.05, **p ≤ 0.01 relative to C57.BL/6 mice, n = 5 representing three individual experiments). WT denotes C57.BL/6 mice. Underlying data are provided in S2 Data.
https://doi.org/10.1371/journal.pbio.3000558.s002
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Reference
- 1. McMahon M, Ye S, Izzard L, Dlugolenski D, Tripp RA, Bean AGD, et al. (2016) ADAMTS5 Is a Critical Regulator of Virus-Specific T Cell Immunity. PLoS Biol 14(11): e1002580. https://doi.org/10.1371/journal.pbio.1002580 pmid:27855162
Citation: McMahon M, Ye S, Izzard L, Dlugolenski D, Tripp RA, Bean AGD, et al. (2019) Correction: ADAMTS5 Is a Critical Regulator of Virus-Specific T Cell Immunity. PLoS Biol 17(11): e3000558. https://doi.org/10.1371/journal.pbio.3000558
Published: November 6, 2019
Copyright: © 2019 McMahon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.