A Dynamical Systems Model for Combinatorial Cancer Therapy Enhances Oncolytic Adenovirus Efficacy by MEK-Inhibition
Figure 6
G1 arrested cells show more potent cell killing effect and virus production.
HCT116 cells were density arrested and released from synchronization. (A) Cell cycle distribution was quantified upon release at 0, 7, 16, and 24-hours. (B) The effect of cell cycle synchronization on CAR expression was analyzed. (C) Cell viability and (D) virus production were measured 3 days after infection (MOI = 1) with WtD, ONYX-014, Delta-24, and Delta-24RGD (denoted as RGD) at 7, 16, and 24-hours after release from density arrest. Error bars represent the standard deviation of triplicate measurements for cell viability and duplicate measurements for virus production.