Shp2 Knockdown and Noonan/LEOPARD Mutant Shp2–Induced Gastrulation Defects
Figure 6
Embryos were coinjected with combinations of suboptimal concentrations of NS-Shp2 (T73I, 75pg or D61G, 100pg), LS-Shp2 (A462T, 50pg or G465A, 30pg), or GFP RNA (150pg). Embryos were assessed at 4 dpf and scored for any of the previously documented phenotypes. Chi2 tests indicate a significant increase in the observed phenotype after coinjection of the two NS-Shp2s or the two LS-Shp2s compared to single NS- or LS-Shp2s with GFP (p < 0.001, double asterisks). Combinations of NS- and LS-Shp2s (single asterisk) are not significantly different from NS- or LS-Shp2 with GFP. The number of embryos that were analyzed is indicated (n).
