Sepsid even-skipped Enhancers Are Functionally Conserved in Drosophila Despite Lack of Sequence Conservation
Figure 6
Extensive reorganization of binding sites between Drosophila and sepsid eve stripe 2 enhancers.
Predicted binding sites for the five factors known to regulate expression from the eve stripe 2 enhancer in six Drosophila species [56] and six sepsid species. Sites were predicted independently in each species using PATSER [61] and mapped onto an MLAGAN [65] multiple alignment of the eve stripe 2 enhancer sequences. The height of the box representing each binding site is scaled by its PATSER p-value (taller boxes represent sites with higher predicted affinities). The top panel (grey shading) shows the positions of biochemically-verified (in vitro footprinting) binding sites [27]. Binding sites conserved within families are indicated by solid boxes. A BCD-KR site pair conserved across families is indicated by a dashed box. Alignment coordinates are indicated.