Transcription Initiation Activity Sets Replication Origin Efficiency in Mammalian Cells
Figure 1
Genomic distribution of ORIs in embryonic stem cells.
(A) ORI distribution at different genomic regions along 10.1 Mb of the mouse genome detected by 300–800 nt long nascent strand hybridisation (n = 97) or both by 300–800 nt and 100–600 nt long nascent strands hybridisation (n = 38). (B) Inter-origin distances along 10.1 Mb of the mouse genome (average = 103 kb; n = 97).(C–E) Distribution of ORIs and promoters in gene-rich versus gene-poor regions. ORI location (C), inter-origin distances (D) and density plots (E), of promoter-rich (chromosome 3 and zone 2 of chromosome X) and promoter-poor regions (zone 1 of chromosome X). The genomic features covered by the array, ORI distribution and percentages of ORI occurrence relative to the annotated genes along the 10.1 Mb and per region examined are summarised in Table S1.