Transcription Initiation Activity Sets Replication Origin Efficiency in Mammalian Cells
Figure 3
Replication initiation activity at CpG island-ORIs.
(A) Array profiles of 9 CpG island-ORIs. Symbols are like in Figure 2.(B) Q-PCR measurements of nascent strands abundance across the positive probes defining the ORIs shown in A in preparations of replication intermediates of the indicated sizes. Primer pairs span less than 2 kb at each region and values were normalised to the average of those obtained at the three negative regions in each gradient fraction. Numbers below each panel indicate fold enrichment of the ORI peak relative to the averaged negative regions. Primer pair sequences are listed in Table S3.