A Forward-Genetic Screen and Dynamic Analysis of Lambda Phage Host-Dependencies Reveals an Extensive Interaction Network and a New Anti-Viral Strategy
Figure 5
The effect of increased phage concentration on infection dynamics.
All of the strains in Cluster 2 and 3 (except ΔcyaA and Δcrr) were tested at three MOIs; the results for the ΔihfA strain are shown in (A) and the rest are found in Figure S2. The bold line indicates the average value of all four measurements. (B) The difference in normalized E. coli concentration between the high and low MOI tests at eight normalized time units is plotted for each strain (The ΔnusB strain time course progressed to only 7 normalized time units due to slow growth but exhibited little difference between MOI tests at that time). (C) For the LPS inner core biosynthesis strains, the high MOI infection growth curves (uninfected in gray and infected in blue) are shown in order of pathway occurrence (top to bottom) to highlight how the dynamics change depending on pathway position. MOI results for strains highlighted with an asterisk were not tested at the higher MOIs and therefore the low MOI results are shown. (D) The time (peak time) and absorbance (peak height) where clearance begins to be detected for each of the time courses shown in (C) is plotted relative to K-12 WT.