A Forward-Genetic Screen and Dynamic Analysis of Lambda Phage Host-Dependencies Reveals an Extensive Interaction Network and a New Anti-Viral Strategy
Figure 8
Determining the function of yneJ.
Single cell analysis of E. coli infection in the presence of GFP expressing lambda phage was performed to assess yneJ's effect on infection rate and the lytic-lysogenic decision. Growth of the infected K-12 WT and ΔyneJ strains were observed at 60× magnification and assessed for GFP expression. (A) Images of K-12 WT and ΔyneJ cells infected with GFP expressing phage. A recently lysed cell can be seen in the bottom left corner of the K-12 WT image. (B) Table summary of data obtained from single cell analysis shows reduced infectivity and no significant change in fraction lytic for ΔyneJ. (C) Bar plot showing the results of quantitative real-time RT-PCR of lamB mRNA, for ΔyneJ as well as several cell lines deficient in known lamB transcriptional regulators. The error bars indicate the standard deviation. The asterisk indicates a fold-change between the K-12 WT and the strain of interest with a p-value ≤0.001.