A Forward-Genetic Screen and Dynamic Analysis of Lambda Phage Host-Dependencies Reveals an Extensive Interaction Network and a New Anti-Viral Strategy
Figure 9
Pathways involved in sulfur metabolism and the thiolation of tRNA nucleosides affect lambda replication both positively and negatively.
Several genes identified in this study fall within tRNA thiolation pathways, as shown schematically here. A star on the secondary structure drawing at right indicates the tRNA modification location. Infection dynamics are displayed (Absorbance (600 nm) vs. Time (hours). The scaling is equivalent for all plots) for knockout strains, demonstrating consistent dynamics within pathways. The pathway that includes ΔtusA, ΔtusBCD, ΔtusE, and ΔmnmA shows dynamics consistent with decreased infectivity (yellow), while the iron-sulfur dependent pathway that goes through IscU shows dynamics consistent with increased infectivity (blue). ΔiscS has a very slow growth rate and is therefore not shown here.