Figures
There is an error in the bond configuration for the linearmycin B structure depicted in Fig 1D. Please view the correct figure here.
(a) When co-cultured on MYM agar, S. Mg1 (left) releases molecule(s) that cause cellular lysis and colony degradation of B. subtilis (PDS0067) (right) at a distance. (b) We cultured B. subtilis (PDS0067) (right) alone on MYM7 agar for 24 h before adding isolated LDA onto a filter paper disc (left) adjacent to the colony, which subsequently lysed over 48 h similarly to co-culture with S. Mg1. (c) HPLC trace of the isolated LDA. The peak is detected by UV absorbance at 333 nm (blue). The background is shown by the 254 nm absorbing trace (red). (d) The structure of linearmycin B. Scale bar is 5 mm.
The images for Figs 6 and 7 are incorrectly switched. The image that appears as Fig 6 should be Fig 7, and the image that appears as Fig 7 should be Fig 6. The figure captions appear in the correct order. In S3 Fig the bottom colony is missing the ΔlytF label. Please view the correct figures here.
Wild type and LDAR mutants were spotted in a perpendicular pattern, cross-wise to each other- B. subtilis (vertical) and S. Mg1 (horizontal). (Left) Strains of B. subtilis with yfiJ+ (PDS0571) have flat, immotile colonies. Proximal to S. Mg1, the colonies are lysed and degraded. (Right) Strains of B. subtilis with the LDAR allele yfiJA152E (PDS0572) develop heterogeneous colonies, having wrinkled surfaces and motile outgrowths. Notably, the colonies of yfiJA152E B. subtilis near S. Mg1 have a distinctive spreading morphology. Plates were photographed after 96 hours co-incubation on MYM + 1.5% agar. Plates represent duplicate experiments.
Genes involved in biofilm formation (epsH, sinR, and degU) and motility (sigD) were deleted from strains with either (a) yfiJ+ (PDS0571) or (b) the LDAR allele yfiJA152E (PDS0572). In all cases, the biofilm and motility mutant strains were sensitive to lysis with wild-type yfiJ and were resistant to lysis with yfiJA152E. All cultures place S. Mg1 on the left and B. subtilis on the right. Colonies were photographed after 72 hours co-incubation with S. Mg1 on MYM medium. Images are representative of triplicate samples. Scale bar is 5 mm.
There is an error in the NCBI BioProject Accession number in the Data Availability Statement and in the RNA-seq section of the Materials and Methods. The correct Accession number is PRJNA295934.
Supporting Information
S3 Fig. Genes predicted to be repressed by YfiK are not responsible for LDA.
Spore-killing factor (SKF) and autolysis were predicted to be regulated by YfiK. Strains of B. subtilis (right) with deletions in genes responsible for SKF biosynthesis (ΔskfA-H) (DL598), an autolysin inhibitor (ΔiseA) (PDS0785), deletions in the major autolysin regulator σD (ΔsigD) (DS323), and deletions in three major autolysins (ΔlytABC, ΔlytD, ΔlytF) (DS2483) were tested for resistance to LDA in co-culture with S. Mg1 (left). All strains lysed similarly to wild type (PDS0066). Cultures were photographed after 72 h co-incubation on MYM agar plates. Scale bar is 5 mm. These results were consistent across six replicates.
https://doi.org/10.1371/journal.pgen.1005807.s001
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Reference
Citation: Stubbendieck RM, Straight PD (2016) Correction: Escape from Lethal Bacterial Competition through Coupled Activation of Antibiotic Resistance and a Mobilized Subpopulation. PLoS Genet 12(1): e1005807. https://doi.org/10.1371/journal.pgen.1005807
Published: January 11, 2016
Copyright: © 2016 Stubbendieck, Straight. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited