Figures
Some ORF names, lengths and mutations in Table 1 are incorrect. Specifically, the ORF names and ORF lengths listed for the paqr-2(et36) and paqr-2(et35) alleles, and the ORF names and mutations listed for the iglr-2(et34), iglr-2(et37) and iglr-2(et38) alleles. The shift by one amino acid position in the mutation column also applies to the iglr-2 mutations indicated in Fig 2. Please see the corrected Table 1 and Fig 2 here.
(A) Schematic structures of the IGLR-2 and PAQR-2 proteins, with novel mutations indicated by red arrowheads. The VC155 and VN173 fragments added to the C and N terminal ends of IGLR-2 and PAQR-2, respectively, allows reconstitution of a full and fluorescent VENUS YFP protein if the two proteins come into close proximity. (B) Result of the BiFC experiment showing that IGLR-2 and PAQR-2 contact each other on cellular membranes. The top two panels show a transgenic worm co-expressing the fusion proteins depicted in (A); note the clear membrane-localized fluorescence indicative of IGLR-2 and PAQR-2 interaction. The middle two panels show a transgenic worm co-expressing the tagged IGLR-2 and a tagged PAQR-1 protein; note that only autofluorescent gut granules emit a signal, indicating that IGLR-2 and PAQR-1 do not interact with each other. The bottom two panels show a transgenic animal carrying the two empty vectors used in the BiFC experiments; note again that only autofluorescent gut granules emit a signal.
Reference
Citation: Svensk E, Devkota R, Ståhlman M, Ranji P, Rauthan M, Magnusson F, et al. (2016) Correction: Caenorhabditis elegans PAQR-2 and IGLR-2 Protect against Glucose Toxicity by Modulating Membrane Lipid Composition. PLoS Genet 12(6): e1006112. https://doi.org/10.1371/journal.pgen.1006112
Published: June 3, 2016
Copyright: © 2016 Svensk et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.