Concerns were raised regarding errors in panel B of S2 Fig. Specifically, the RT-PCR gel electrophoresis results were duplicated for At3g06830 and At1g73630, and for At3g01820 and At2g28080. The authors determined the source of the errors that were made in the preparation of S2 Fig and have provided a corrected figure here.
Supporting Information
S2 Fig. RT-PCR analysis of gene expression.
Total RNA from indicated tissues—dry pollen, 0.5 h PT, 4 h PT, 8- and 21-day-old seedlings (DS)—was used as templates to perform oligo-dT primed reverse transcription reactions followed by cDNA synthesis. RT-PCR was performed with cDNAs from indicated tissues and gel images of PCR products amplified are shown. (A) RT-PCR analysis of pollen-enriched and pollen-expressed genes. (B) RT-PCR analysis of genes that are significantly altered in SIV PT compared to 4 h PT. (C) RT-PCR analysis of pistil-dependent gene expression changes in vivo. Samples analyzed were dry pollen, unpollinated ms1 pistils (virgin pistil), ms1 pistils pollinated for one minute (1 m pollinated pistil) and ms1 pistils pollinated for two hours (2 h pollinated pistil).
https://doi.org/10.1371/journal.pgen.1006210.s001
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Reference
Citation: Qin Y, Leydon AR, Manziello A, Pandey R, Mount D, Denic S, et al. (2016) Correction: Penetration of the Stigma and Style Elicits a Novel Transcriptome in Pollen Tubes, Pointing to Genes Critical for Growth in a Pistil. PLoS Genet 12(7): e1006210. https://doi.org/10.1371/journal.pgen.1006210
Published: July 19, 2016
Copyright: © 2016 Qin et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.