Differentiation of Human Embryonic Stem Cells to Regional Specific Neural Precursors in Chemically Defined Medium Conditions
Figure 6
(A) Response to 100 µM glutamate (Glu) and blockade of the current by the simultaneous application of the antagonist CNQX (n = 11). The horizontal bar indicates the duration of agonist (red line) and/or antagonist (blue line) or modulator (green line) application. (B): Response of cells to 100 µM of GABA and blocking of the current with the specific GABAA receptor antagonist bicuculline (Bic) (n = 25). (C): Reversible downregulation of Glu currents in cells treated with bFGF by applying 1 mM of the metabotropic neurotransmitter dopamine (DA) for two minutes to the cells (n = 8). (D): Response of cells treated with RA to 100 µM acetylcholine (Ach) (n = 8). The current was fully blocked with 100 µM of (−) tubocurarine (Cur) (n = 6).