Unphosphorylated SR-Like Protein Npl3 Stimulates RNA Polymerase II Elongation
Figure 1
Npl3 stimulates RNAP II elongation.
Oligo(dC)-tail templates were used in transcription reactions with or without 78 nM Npl3, as indicated. Reactions were pulsed by the addition of ATP, GTP, and α32P-labeled CTP for 30 minutes as described in Materials & Methods. Transcripts were chased by the addition of excess CTP and limiting UTP for the indicated times. A schematic of the experimental scheme is shown on top. The oligo(dC)-template is represented next to the gel and the positions of the pause sites at stretches of Ts are indicated.