Genomic Androgen Receptor-Occupied Regions with Different Functions, Defined by Histone Acetylation, Coregulators and Transcriptional Capacity
Figure 2
DHT-responsive genes in C4-2B cells.
Illumina expression arrays were used to measure expression levels of 46,713 transcripts in three replicates before and three replicates after DHT exposure in C4-2B cells, including the 1,232 RefSeq transcripts within our chromosome 19/20 genome tiling arrays, which are shown here. The student's t-test was used to determine statistical significance, and p-values were adjusted based on random permutations of the full dataset. This volcano plot shows the E-value (number of transcripts at the given p-value expected by chance) plotted against the mean fold change. 24 transcripts up-regulated at the E = 5 level (permutation-adjusted p-value = 0.003) and 19 transcripts down-regulated, are shown in the upper two quadrants. Transcripts are color-coded based on whether they are adjacent to an AROR, and the up-regulated transcripts show an elevated number adjacent to acetylated, but not un-acetylated, ARORs. The inset shows a time course of endogenous gene expression. C4-2B cells were cultured in hormone-depleted medium for 3 days and then treated with DHT (10 nM) or ethanol vehicle for the indicated times. Expression levels of 4 representative genes were measured by real-time RT-PCR. The data is normalized to 18S expression in log scale; Values are fold changes over the vehicle control at each time point.