Genomic Androgen Receptor-Occupied Regions with Different Functions, Defined by Histone Acetylation, Coregulators and Transcriptional Capacity
Figure 3
Histone H3 acetylation defines a distinct subclass of ARORs.
Histone H3 acetylation peaks overlap 52% of the most reproducible L1 ARORs, but only 27% of the L2 ARORs, 12% of the L3 ARORs, and 6% of random sequences from the area covered by the chromosome 19/20 tiling array (A). Transcripts adjacent to acetylated ARORs are significantly more likely to be up-regulated in C4-2B cells, with 12.5% of transcripts showing up-regulation, as opposed to 1.1% of those adjacent to un-acetylated ARORs, and 1.1% of those not adjacent to any AROR; this was not the case for repressed genes (B). While un-acetylated L1/L2 ARORs are about as likely as randomized controls to cluster on the genome as shown by the cumulative distribution plot of inter-AROR distances (C), acetylated ARORs show significantly more genomic clustering (D).