Genomic Androgen Receptor-Occupied Regions with Different Functions, Defined by Histone Acetylation, Coregulators and Transcriptional Capacity
Figure 4
Most ARORs have enhancer potential (transactivation activity), which is associated with several sequence motifs.
Transient transfection luciferase reporter assays were carried out in duplicate, and repeated independently at least 3 times for 61 of 62 L1 ARORs before and after DHT exposure, and the results are displayed in a volcano plot (4A) which shows the fold change between DHT+ and DHT- luciferase levels and the p-value significance level by the Student's t-test. At the p<0.05 level, 19 ARORs (31%) were strongly induced, while 21 ARORs (34%) were weakly induced. From L1 ARORs, 52 sequence motifs from Transfac and de novo motif discovery algorithms were found to be significantly enriched (see text). We performed chi-square tests to determine those motifs (B, upper) and motif pairs (B, lower) significantly enriched in the 18 strongly induced (red bars) vs. the 19 non-responsive (blue bars) ARORs. Four motifs were identified (C), including binding sites for FoxA1, the AR, along with two de novo motifs with similarity to FoxA1, NFI, and Oct1.