Genomic Androgen Receptor-Occupied Regions with Different Functions, Defined by Histone Acetylation, Coregulators and Transcriptional Capacity
Figure 6
Expression of AR target genes after siRNA-mediated knockdown of coregulators.
C4-2B cells were transfected with siRNA targeting AR, FoxA1, NFI, CEBPβ, GATA2 or non-specific (NS) regions. Two days after transfection, cells were treated with 10 nM DHT or ethanol vehicle for 18 h. Expression levels of five AR target genes were examined by real-time RT-PCR. Relative mRNA level was normalized by GAPDH mRNA. Efficiency of each siRNA knockdown was measured by immunoblot with indicated antibodies (upper right).