Metnase Mediates Resistance to Topoisomerase II Inhibitors in Breast Cancer Cells
Figure 2
Metnase shRNA expression induces metaphase arrest in MDA-MB-231 cells.
(A) Metnase expression was reduced by shRNA expression in MDA-MB-231 cells and analyzed by RT-PCR of mRNA and Western blot of protein, with 18S RNA and β-actin levels as loading controls. (B) Quantification of metaphase arrest after treatment with 2 or 10 µM ICRF-193 for 18 hrs. The percentages of metaphase cells in DMSO control cultures were subtracted from ICRF-193 treated cultures. Values are averages (+SEM) for 10–15 determinations; single asterisk indicates P = 0.022 and double asterisk indicates P = 0.0002 (t tests). (C) Representative images of control (shGFP) and Metnase knockdown cells expressing treated with ICRF-193 and stained with α-tubulin (green) and DAPI; arrows indicate metaphase cells.
