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Behavioral, Medical Imaging and Histopathological Features of a New Rat Model of Bone Cancer Pain

Figure 7

Hematoxylin & eosin coloration and quantification of TRAP staining in the rat distal femur at day 21 post-surgery.

(A–B) Photomicrograph representative of the sham surgery group showing healthy bone structures (BM = Bone Marrow; SB = Spongy Bone). (C–D) Cancer-implanted bone illustrating an unstructured architecture. Note that the cartilage is still unaffected (arrows). Hematopoietic cells from the bone marrow are entirely replaced by proliferating MRMT-1 cells (Tu). Necrotic clusters (NC) appear in early areas of proliferation. Cancellous bone is sparsely deposited in a mosaic pattern, yielding fragile and disorganized bone. Larger lacunae and immature bone are indications of compensatory reconstruction. (E–F) Photomicrographs of trabecular bone stained with the TRAP coloration technique. Multinucleated active osteoclasts, bordering the hydroxyapatite matrix, are stained in brown-red (black arrows) in sham (E) and cancer-bearing (F) rats. (G) The number of differentiated osteoclasts per mm2 is three times greater in cancer rats than in sham animals, explaining the accelerated degradation of bone structure. ***: p≤0.001 (n = 12 for sham and n = 20 for the cancer group). Scale bars A,C: 1 mm; B,D,E,F: 90 µm.

Figure 7

doi: https://doi.org/10.1371/journal.pone.0013774.g007