Alcohol Exposure Decreases CREB Binding Protein Expression and Histone Acetylation in the Developing Cerebellum
Figure 6
AcH4 expression was decreased in the cerebellum of ethanol-exposed developing rats.
(A) Image of one of the western blots showing differential acetylated H4 (AcH4) expression in the cerebellum isolated from control pups (exposed to air) analyzed at P2 to P12. (B) Representative AcH4 western blot analysis of cerebellum isolated from ethanol-exposed pups analyzed at p2 to p12. (C) Quantitative analysis showing that AcH4 protein levels (normalized to b-actin) are lower in the ethanol group compared to the control group between P2 and P10, but not at P12. Three independently exposed liters were analyzed and each was counted as n = 1. Multiple western blots were analyzed for each exposure to minimize the variation between western blots. (D) Representative total H4 (Total H4) western blot analysis of cerebellum isolated from control pups (exposed to air) analyzed at P2 to P12. (E) Representative Total H4 western blot analysis of cerebellum isolated from ethanol-exposed pups analyzed at P2 to P12. (F) Quantitative analysis showing that total H4 protein levels (normalized to b-actin) are not changed in the ethanol group compared to the control group. (G) Quantitative analysis showing that the ratio of AcH4 compared to total H4 protein levels are lower in the ethanol group compared to the control group between P2 and P10, but not at P12. (H) Representative AcH4 immunohistological images of control and ethanol-exposed cerebella at P8. AcH4, red; Dapi, blue. Scale bar = 50 µm. (I) Quantitative analysis of AcH4 immunohistological signal intensity demonstrates that AcH4 expression is reduced in the cerebellum of ethanol exposed rats compared to controls. N = 4 individual animal/condition. P<0.05 t-test.