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Plexin-B1 Activates NF-κB and IL-8 to Promote a Pro-Angiogenic Response in Endothelial Cells

Figure 5

Sema4D produced by tumor cells activates NF-κB in endothelial cells.

A. Immunoblot analysis for Sema4D on lysates from HN13 cells, infected with empty vector control lentivirus (control) or virus coding for Sema4D shRNA (S4DshRNA). GAPDH was used as a loading control (lower panel). B. Immunoblot for phospo- (upper panel) and total (middle panel) I-κB in HUVEC growing in media conditioned by control infected HN13 cells (control, left column) or cells infected with Sema4D shRNA expressing lentivirus (S4DshRNA, right column) for the times indicated. GAPDH was used as a loading control (lower panel). C. Immunofluorescence on tumor xenografts composed of control infected HN13 cells or cells infected with Sema4D shRNA, for CD31 (endothelial cells, red) and p65 (green). The white arrows indicate endothelial cell nuclei. D. The results of the xenograft immunofluorescence expressed as the percentage of p65 positive endothelial cell nuclei counted in 10 high power fields (*, p<0.05).

Figure 5

doi: https://doi.org/10.1371/journal.pone.0025826.g005