Activation of Sirt1 by Resveratrol Inhibits TNF-α Induced Inflammation in Fibroblasts
Figure 5
mTOR/S6RP is also involved in the inhibitory function of resveratrol on TNF-α induced inflammation.
A. 3T3/NIH cells were treated with TNF-α (10 ng/ml) for 0, 1/2, 3, 9, 24 h, phosphorylation of mTOR and S6RP was detected by western blotting analysis. B. Cells were pretreated by rapamycin (Rapa, 10 ng/ml) or resveratrol (50 nM) for 1 h in the presence of TNF-α treatment for 24 h, MMP-9 released in medium was measured by gelatin zymography. C. Cells were processed as (B) for 3 h, IL-1β and phosphorylation of mTOR and S6RP were examined by western blotting analysis in cell lysis.