Hypoxia-Adaptation Involves Mitochondrial Metabolic Depression and Decreased ROS Leakage
Figure 3
Effect of adaptation to hypoxia on mitochondrial respiratory chain complex activities.
The enzymatic activity of each respiratory chain complex was measured in isolated mitochondria as described in the Methods section. (A) Complex I: no significant changes in complex activity was observed (n = 3, p>0.05) between controls (NF) and hypoxia-adapted flies (AF). (B) Complex II: significant down-regulation of complex activity was determined in complex II in the hypoxia-selected flies (AF) (n = 3, p<0.05). (C) Complex III: significant up-regulation of complex activity in the hypoxia-selected flies (AF) (n = 3, p<0.05). (D) Complex IV: significant up-regulation of complex activity in the hypoxia-selected flies (AF) (n = 3, p<0.05). All activities are presented as percent of the naive control values (mean ±SEM).