Induction of Selective Blood-Tumor Barrier Permeability and Macromolecular Transport by a Biostable Kinin B1 Receptor Agonist in a Glioma Rat Model
Figure 6
Systemic infusion of NG29 increases permeability and uptake of albumin within peritumoral tissue.
(A) Direct immunological staining of endogenous albumin in brain tissues from F98-implanted rats treated or not with intracarotid NG29 (50 nmol/kg/min for 5 min). Representative coronal sections of CTL-, vehicle- and NG29-treated rats immunostained with sheep anti-rat albumin HRP conjugated are shown in the upper panels. Scale bar: 1 mm. Histographic representation of integrated optical density (IOD) values (left) and stained surface areas (right) of immunoreactive albumin in respective animal groups (bottom panels). ***p<0.001 vs CTL. (B) Semi-quantitative measurement of Evans blue content (mg/g wet weight tissue) in several tissues after systemic intravenous (femoral) injection of saline vehicle or NG29 (5 µmol/kg i.v.) in F98 glioma bearing rats. Data are presented as means ± S.E.M. n = 5 to 7 animals per group. *p<0.05 vs respective vehicle group.