Cell Recruitment and Cytokines in Skin Mice Sensitized with the Vaccine Adjuvants: Saponin, Incomplete Freund’s Adjuvant, and Monophosphoryl Lipid A
Figure 5
Leukocyte immunophenotypic profile in peripheral blood of mice sensitized with vaccine adjuvants: saponin (SAP; light gray), incomplete Freund’s adjuvant (IFA; medium gray), and monophosphoryl lipid A (MPL; dark gray) at 24 or 48 h after stimulation.
The control group was inoculated with 0.9% sterile saline (C; white). The bar graphs present the percentage of cells expressing CD14+ (monocytes), CD19+ (B lymphocytes), and CD4+ and CD8+ (T-lymphocyte subsets). Significant differences (p<0.05) between groups are represented by the letters a, b, c and d, referring to the C, SAP, IFA, and MPL groups, respectively, and ANOVA following Tukey’s test was employed. Data presented are the mean±SD from groups of five animals/evaluation time.