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Novel RNAi-Mediated Approach to G Protein-Coupled Receptor Deorphanization: Proof of Principle and Characterization of a Planarian 5-HT Receptor

Figure 8

RNAi-based DtSER-1 deorphanization.

Treatment groups are Control (control dsRNA) and DtSER-1 RNAi (DtSER-1 dsRNA). Treatments are C (control) and 5-HT (10 M). Each bar is the mean ( SEM) of 3 individual experiments. cAMP levels were normalized to basal cAMP levels (set at 100%), and the datasets were joined. DtSER-1 knockdown corresponds to significantly decreased cAMP stimulation (∼32%) in response to 5-HT. Analysis of the raw cAMP values of individual experiments renders the same results (Table 3). Asterisks indicate significance at P0.001 (***), and “ns” indicates no significant difference (one-way ANOVA, Tukey post hoc test).

doi: https://doi.org/10.1371/journal.pone.0040787.g008