Influence of Galloyl Moiety in Interaction of Epicatechin with Bovine Serum Albumin: A Spectroscopic and Thermodynamic Characterization
Figure 8
ITC profiles of BSA when titrated with catechins (T = 298 K).
The top panels (A) and (B) present raw results for the sequential injection of EC (500 µM) and ECG (500 µM) into BSA solution (50 µM) and dilution of catechins into buffer (curves on the top offset for clarity). The lower panels show the integrated heat results after correction of heat of dilution against the mole ratio of Catechin/BSA. The points (closed squares) were fitted to a one-site model and the solid lines represent the best-fit results.