High Fat Diet-Induced Gut Microbiota Exacerbates Inflammation and Obesity in Mice via the TLR4 Signaling Pathway
Figure 7
Effect of HFD on NF-κB activation, Akt and FOXO3a phosphorylation, and tight junction–associated protein levels in the colon of TLR4-deficient mice.
(A) Western blot analysis for iNOS, COX-2, p-IKKβ, p-p65 occludin, and claudin-1 protein levels (left) and quantification of western blot (right) in the colon lysates from TLR4-deficient mice are shown. (B) Western blot analysis for p-p65,p-65, p-Akt, Akt, p-FOXO3a, and FOXO3a protein levels (left) and quantification of western blot (right) in the colon lysates from wild-type or TLR4-deficient mice are shown. All values were indicated as the mean ± SEM (n = 5).