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Open Access

Peer-reviewed

Research Article

Mast Pulses Shape Trophic Interactions between Fluctuating Rodent Populations in a Primeval Forest

Abstract

How different functional responses of consumers exploiting pulsed resources affect community dynamics is an ongoing question in ecology. Tree masting is a common resource pulse in terrestrial ecosystems that can drive rodent population cycles. Using stable isotope (δ13C, δ15N) analyses, we investigated the dietary response of two fluctuating rodent species, the yellow-necked mouse Apodemus flavicollis and the bank vole Myodes glareolus, to mast events in Białowieża Forest (NE Poland). Rodent hair samples were obtained non-invasively from faeces of their predators for an 11-year period that encompassed two mast events. Spectacular seed crops of deciduous trees, namely oak Quercus robur and hornbeam Carpinus betulus, occur after several intermediate years of moderate seed production, with a post-mast year characterised by a nil crop. While a Bayesian isotopic (SIAR) mixing model showed a variety of potential vegetation inputs to rodent diets, the isotopic niche of the yellow-necked mouse was strongly associated with mast of deciduous trees (>80% of diet), showing no variation among years of different seed crop. However, bank voles showed a strong functional response; in mast years the vole shifted its diet from herbs in deciduous forest (∼66% of diet) to mast (∼74%). Only in mast years did the isotopic niche of both rodent species overlap. Previous research showed that bank voles, subordinate and more generalist than mice, showed higher fluctuations in numbers in response to masting. This study provides unique data on the functional response of key pulse consumers in forest food webs, and contributes to our understanding of rodent population fluctuations and the mechanisms governing pulse–consumer interactions.

Citation: Selva N, Hobson KA, Cortés-Avizanda A, Zalewski A, Donázar JA (2012) Mast Pulses Shape Trophic Interactions between Fluctuating Rodent Populations in a Primeval Forest. PLoS ONE 7(12): e51267. https://doi.org/10.1371/journal.pone.0051267

Editor: Wayne M. Getz, University of California, Berkeley, United States of America

Received: August 21, 2012; Accepted: October 30, 2012; Published: December 10, 2012

Copyright: © 2012 Selva et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This study was partly supported by the Polish-Spanish joint research projects CSIC-PAS (2006PL0013 and 2008PL002), by the Polish Ministry of Science and Higher Education (project NN304294037) and an operating grant by Environment Canada to KAH. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

Introduction

Resource pulses, defined as brief and infrequent events of high resource availability, are widespread phenomena in nature that have tremendous impacts on consumer communities, including bottom-up effects with consequences for multiple trophic levels [1]–[3]. One of the most common types of pulsed resources in terrestrial ecosystems is mast seeding, especially among temperate tree species [1]. Masting is the synchronous intermittent production of large seed crops by a population of plants [4]. It occurs after several intermediate years of moderate seed production, and is followed by a post-mast year often characterised by a nil crop. In deciduous forests, masting triggers rapid growth in rodent densities which is typically followed by a population crash, thus initiating a sequence of effects which propagate throughout the food web [1], [5]–[8]. How resource pulses elicit different responses of consumers and how this, in turn, affects community dynamics through competitive interactions are ongoing questions in ecology [2].

Pulsed resources affect consumer demography and resource use [1], [2]. Besides food availability, trophic interactions, parasites and predation have been shown to also play a role in rodent population dynamics [6], [9], [10]. In particular, the role of interspecific competition in shaping the structure and dynamics of rodent communities is of considerable interest [11], [12]. Niche divergence or resource partitioning are a key mechanism that can reduce conflicts among similar species with overlapping niche requirements. In this sense, niche overlap is often interpreted in light of potential competition [13]–[16]. However, documenting competition in natural communities is difficult due to complexities associated with past competition and challenges related to the quantification of resource limitation and their use by consumers at the individual and population level [12], [13], [16], [17]. Masting events provide a natural experiment in resource manipulation to investigate how resource pulses mediate competitive and trophic interactions among consumer species. Although the numerical response of consumers to pulses has been widely documented [1], [2], few studies have additionally included evidence of these consumers using the pulsed resource [18].

Here, we used a stable isotope approach to investigate the dietary response of two sympatric and ecologically similar rodent species to mast cycles (consisting of mast, post-mast and intermediate years) in the Białowieża primeval forest (NE Poland). This approach allowed us to avoid some of the shortcomings of tracing nutrient flow through animal communities using conventional dietary analysis techniques [19]. The isotope approach is particularly amenable when the pulsed resource differs isotopically from other available foods [20], [21] and when exploring the generalized ecological niche of co-occurring individuals and populations [22].

Our main objective was to investigate trophic segregation between two rodents, the bank vole Myodes glareolus and the yellow-necked mouse Apodemus flavicollis, by tracing the consequences of superabundant food pulses provided by mast events. Both species follow population fluctuations triggered by masting; their numerical response has been well documented [5], [6], [23]. We hypothesized that masting will cause significant changes in the stable isotope (δ13C, δ15N) composition of both species. According to ecological theory [16], [17], during mast events we expected a dietary and isotopic convergence of the two species. We anticipated that if interspecific competition for food resources was important, during non-mast years the species would differ isotopically due to trophic or dietary segregation. We expected a functional response of both species to mast cycles, with larger variation in the food niche of bank voles, the smaller and subordinate species [12], [24].

Materials and Methods

(a) Ethics Statement

All necessary permits were obtained for the described field studies. Samples did not belong to any protected species. The Białowieża National Park administration issued the permit for the collection of vegetation samples in this protected area; no permit was required for sample collection in the commercial unprotected part of the forest. The field studies did not involve endangered or protected species. The Mammal Research Institute in Białowieża gave permit to access its collection; rodent specimens were loaned to collect hair samples.

(b) Study Area

Białowieża Forest is a well-preserved lowland temperate forest located in NE Poland, at the Polish-Belarusian border. The most primeval part is the Strict Reserve of the National Park, dominated by oak- lime-hornbeam (Quercus robur-Tilia cordata- Carpinus betulus) forest [6]. Spectacular and synchronized seed crops of oak and hornbeam occur at 6–9 year intervals, followed by a year of nil crop, and moderate crops in the intervening years. In the autumn of mast years, the biomass of seeds produced by pristine oak-lime-hornbeam stands can be up to almost 2000 kg/ha, in comparison to c.a. 300 kg/ha of seeds produced on average in intermediate years [5]. The community of forest rodents, dominated by bank voles and yellow-necked mice, represents a main mast consumer. Their populations peak in autumn of the year after the mast (post-mast year) and crash afterwards [5], [6].

(c) Sample Collection

Plant samples (N = 161, representing 42 species or groups, Table S1) were collected in 2007–2011 in an area of about 160 km2, including the Strict Reserve of the Białowieża Forest, the most primeval, and its surrounding area. We collected fruits and seeds from different individual plants, mainly trees, and, in the case of herbaceous vegetation, leaves from those species most abundant and representative of the main habitat/forest types (deciduous forest, coniferous forest, alderwoods and meadows).

Rodent hair samples (N = 110) were obtained non-invasively from the remains of predator faeces collected during previous diet studies in the primeval part of the forest [25] and stored at the museum of the Mammal Research Institute in Białowieża. Hair samples for isotope analysis were selected to maximize the representation of mast, post-mast and intermediate years, and of adult individuals. We focused on an 11-year period (1986–1996) that encompassed the oak and hornbeam masting of autumn 1989 and 1995 [5], [6]. All samples belonged to the period October-April, which corresponded to rodent hair grown in autumn. Hair samples were classified as corresponding to “mast” years, “post-mast” years (the year following the mast, with nil crop), and “intermediate” years of moderate seed production (neither mast nor post-mast year).

(d) Stable Isotope Analysis

Plant material was washed in distilled water, dried, and powdered prior to isotope analysis. Hair was first rinsed in a 2∶1 chloroform:methanol solution to remove surface oils and cut into small pieces. Samples were combusted in a Eurovector 3000 (Milan, Italy–http://www.eurovector.it) elemental analyzer. The resulting CO2 and N2 analyte gas from the samples was separated by Gas Chromatograph (GC) and introduced into a Nu Horizon (Nu Instruments, Wrexham, UK–http://www.nu-ins.com) triple-collector isotope-ratio mass-spectrometer via an open split and compared to a pure CO2 or N2 reference gas. For hair, the Bowhead Whale baleen standard BWB II (δ13C = −18.5‰, δ15N = 14.4‰) and porcine (PRC) gelatin (δ13C = −13.5‰, δ15N = 4.69‰) laboratory standards were used. For plant material, we used an-house peagrain standard (δ13C = −24.7‰, δ15N = 2.4‰). Measurement errors based on within-run measurements of standards (SD) were better than ±0.2‰. All stable isotope ratios were reported in the delta notation as parts per thousand (‰) deviation from an appropriate international elemental standard according to the equation:where X is the heavy isotope (13C, 15N), RA is the isotopic ratio (13C/12C, 15N/14N) of the sample and RS is the isotope ratio of the international standard (δ13C: Vienna Pee Dee Belemnite carbonate (VPDB); δ15N: atmospheric nitrogen (AIR)).

(e) Statistical Analysis

We were interested in whether or not mast from individual tree species could be identified isotopically in order to trace mast pulses through small-mammal communities. Our a priori assumption was that plant communities would segregate according to the four main habitat/forest types in Białowieża Forest [6]: (a) deciduous-dominated forest (oak-lime-hornbeam forest); (b) coniferous-dominated forest, composed mainly of Norway spruce Picea abies and Scots pine Pinus silvestris, (c) alderwoods and streamside forest, dominated by black alder Alnus glutinosa and (d) meadows, with sedges, grasses and forbs. Spruce admixtures are common in all forest types. We used MANOVA and a Tukey post-hoc analyses to test among vegetation categories. Plant samples represented 42 species or groups (Table S1). From these isotopic analyses we considered 18 vegetation categories relevant to rodent diets (F17,136 = 11.7, p<0.001). Tukey’s post-hoc tests identified 4 homogeneous subsets for δ15N and 5 subsets for δ13C. These corresponded to 8 vegetation groups which could be distinguished based on at least one isotope (Table 1). Those vegetation groups were portrayed (excluding lichens) in bivariate (δ13C and δ15N) space in preparation for a mixing model analysis (Figure 1). The contributions of isotopically distinct dietary endpoints to the diets of the two species were then investigated further using the Bayesian mixing model SIAR [26] (Table S2) in R.2.10.1.

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Figure 1. Convex mixing polygon (excluding lichens) used to model potential rodent dietary inputs.

Vegetation groups (green circles) correspond to those listed in Tables 1 and S2. Triangles are bank voles and circles are yellow-necked mice (see Figure 2 for corresponding intermediate, mast and post-mast categories depicted).

https://doi.org/10.1371/journal.pone.0051267.g001

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Figure 2. Isotope values of the rodent species along mast cycles.

Mean (± S.E.) δ15N and δ13C values for bank voles (grey symbols) and yellow-necked mice (black symbols) in mast, post-mast and intermediate years. Also shown are positions animals would occupy if they had exclusive diets of deciduous forest herb vegetation and mast seeds (oak, hornbeam, lime, spruce).

https://doi.org/10.1371/journal.pone.0051267.g002

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Table 1. Isotopically distinct vegetation groupings (mean±SE) used to examine dietary niches of the two rodent species in Białowieża Forest.

https://doi.org/10.1371/journal.pone.0051267.t001

The δ13C and δ15N values of rodent hairs were compared among year categories and species using analysis of variance (ANOVA). We examined how the predicted isotopic values of the diet of the rodent species (Table S3) fit within the vegetation isotope space by first applying diet-hair isotopic discrimination factors (Δ13C and Δ15N) to our measured rodent hair isotope values. Precise discrimination values are not well understood and are known to vary according to taxonomic group and diet quality [27]. While Miller et al. [28] and De Mots et al. [29] determined Δ13C discrimination value of +0.3‰ and a Δ15N of +3.3‰ for Peromyscus, these values did not provide dietary values falling within the expected isotope foodweb space for our study area primarily due to inappropriately low values of Δ13C. So, we instead used a Δ13C discrimination factor of +3.2‰ associated with pure herbivores derived by Sponeheimer et al. [30], as summarizes in Table S2. Larger Δ13C have similarly been found for voles raised in captivity on experimental diets differing in protein content by Sare et al. [31]. We did not measure protein content of potential foods for the two rodent species in our study site, but note that the %N composition of all plants measured ranged between 0.2 to 7.0%. While we cannot be sure of precise isotopic discrimination factors corresponding to the voles in our wild population, the excellent fit of our rodent hair isotope values within the convex mixing polygon (Figure 1) gives us confidence that the values we have used are reasonable. In addition, regardless of discrimination factors used, the functional response of these rodents to changes in mast abundance in bivariate isotopic space was of primary interest.

Results

We recorded a broad range of isotope values of potential plant species and tree seeds in the Białowieża Forest (Table S1), which converged into eight isotopically distinct groups (Table 1). After applying appropriate isotopic discrimination factors linking hair to diet, each species fell within a convex polygon in bivariate (δ13C, δ15N) dietary space (Figure 1), suggesting that our choice of potential dietary items encompassed those actually used. Both species were located primarily along a trajectory between the two strong dietary candidates including seeds of oak, hornbeam, lime and Norway spruce at one end and herbs associated with deciduous forest and graminoids at the other (Figure 2). Assuming only a two-source, two isotope mixing model to derive potential use of deciduous forest herbs vs. mast, we found that while mice depended largely on mast throughout the period of investigation, voles shifted from herbs to mast in mast years (Table 2, Figure 2).

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Table 2. Predicted (mean and 95% probability) contributions of mast (from oak, hornbeam, lime, spruce isotopic complex) to the diets of the yellow-necked mouse and bank vole through masting cycles assuming only a (SIAR) two-source (deciduous forest herbs vs. mast) mixing model corresponding to the depiction in Figure 2.

https://doi.org/10.1371/journal.pone.0051267.t002

Hair δ15N values for bank voles were significantly different among mast, post-mast and intermediate years (F2,66 = 8.18, p<0.001, Figure 2, Table S3). However, the δ13C value of vole hairs did not vary in relation to mast events (F2,65 = 1.20, p = 0.308, Figure 2, Table S3). The isotopic diet of the yellow-necked mice did not change significantly among years of different seed crop (δ13C: F2,38 = 1.23, p = 0.304; δ15N: F2,38 = 0.22, p = 0.807) and was strongly associated with a vegetation group that involved mast in all years (Tables 2, S2). In general, bank vole hairs had lower δ13C values (F1,107 = 6.55, p<0.05) but higher δ15N values (F1,108 = 11.67, p<0.001) than those of yellow-necked mice (Figure 2, Table S3).

Discussion

This study provides exceptional quantitative data on the different dietary response of two consumers to resource pulses. The isotopic dietary niche of both rodents did not overlap in post-mast and intermediate years; however, in mast years, their diets clearly converged and showed isotope values consistent with those of deciduous mast. The yellow-necked mouse diet was generally consistent with one mainly of seeds and did not vary among years. However, the bank vole apparently switched its diet according to food availability and consumed primarily herbs in non-mast years. Previous conventional dietary studies indicate that mice species are fundamentally seed-eaters, while voles mainly consume herbs [32]–[34]. Interestingly, the bank vole has been shown to occupy an intermediate position between mice and other vole species [34], supporting its generalist character shown in this study.

Stable isotope analysis allowed us to obtain direct quantitative evidence for dietary convergence between the rodent species during mast events. However, isotopic similarity between oak, hornbeam, lime and Norway spruce did not allow us to distinguish among these masting trees. While the Bayesian mixing models will always fit a solution to the input data, we note that such models become more informative when coupled with other dietary information that can constrain the range of possible solutions. In our case, it would be particularly interesting to know how subtle isotopic changes in rodent tissues measured through masting cycles translate into actual dietary shifts. Potentially, other stable isotopes (δ34S, δ2H) or forensic markers (e.g. compound specific isotopic markers) will allow hornbeam and oak inputs to diets of small mammals to be resolved in future studies. Our study provides additional evidence to previous research on rodent population dynamics suggesting that oak and hornbeam masting trigger rodent outbreaks in Białowieża Forest [5], [6], [23] Our study highlights the potential of stable isotopes in the study of resource pulses. Stable isotopes have been used to investigate the long-term use of hoarded pulsed resources [20], the trophic shift of pulse consumers [35] or the influence of marine [18], [36] and terrestrial subsidies [15] on animal populations. However, they have been rarely used to provide empirical evidence of resource pulse use in studies dealing with numerical responses of primary and secondary pulse consumers [18]. Our results well complement the findings of the long-term research on rodent fluctuations in Białowieża Forest [5], [6], [23] and may be inspiring for further research on mast-driven rodent population dynamics and their consequences in the ecosystem. The present study also reveals the potential of faeces and pellets of predators as a source of samples for isotope analysis, of special interest when covering long periods of time.

Although masting is widely recognised as the main factor triggering population outbreaks in mast consumers in temperate deciduous forests [1], [2], [5]–[8], [23], factors associated with the population crash are still debatable [9], [37]. In fact, food competition has been pinpointed as one of the mechanisms acting in a density-dependent way in rodent population dynamics in Białowieża Forest [23]. Our results show that the two species showed a different functional response to the seed pulse, providing insights into how resource fluctuations may influence competitive interactions. Winter reproduction occurs in both species just following the mast, which indicates low intra- and interspecific competition in that period [5]. In the post-mast year, which coincides with the maximum densities of rodents and the beginning of the population crash [5], the species clearly segregate. It can be hypothesized that during periods of food shortage the most dominant species, the yellow-necked mouse [24], likely monopolise mast, the most valuable food, forcing the bank vole to exploit other food resources and diversify its dietary niche. Mice may experience stronger variations in intraspecific competition, which may also be highest during shortage periods (post-mast years). Bank voles may consume mast only in periods of high availability, when foraging costs, including those associated with the presence of competitors, are low. Although their numerical response is similar, fluctuations in vole numbers are much higher than in yellow-necked mice [5], [6], [23] suggesting also that the numerical response to the pulse of the generalist species was stronger. In summary, our results demonstrate that stable isotope analysis are a reliable approach to track nutrient flow in pulsed resources scenarios and encourage to investigate the role that competitive interactions, mediated by resource pulses, may play in rodent fluctuations in forest dominated by mast-producing trees.

Supporting Information

Table S1.

Summary isotope data (mean±S.E.) of Białowieża Forest vegetation samples analyzed in this study, 2007–2011 (N = 161). When sample size is <3, original values are given.

https://doi.org/10.1371/journal.pone.0051267.s001

(DOC)

Table S2.

Predicted mean contributions of vegetation groups to diets of yellow-necked mice and bank voles based on SIAR mixing model corresponding to the convex polygon shown in figure 1. For illustrative purposes, to demonstrate the largely non informative nature of the mixing model whereby consumer tissues data fall generally centrally within the mixing bivariate spec, we only present results of overall means using the siarsolomcmcv4 command in SIAR. Vegetation endpoints are: 1. Coniferous forest ground vegetation, 2. Deciduous forest ground vegetation, 3. Vaccinium sp., 4. Pinus sylvestris seeds, 5. Corylus avellana seeds, 6. Quercus robur/Carpinus betulus/Tilia cordata/Picea abies seeds, 7. Mushrooms.

https://doi.org/10.1371/journal.pone.0051267.s002

(DOC)

Table S3.

Stable-carbon and nitrogen isotope values (mean±S.E.) of hairs from yellow-necked mice and bank voles in years of different seed crop, and predicted diet isotopic composition after applying diet-hair discrimination factors (see main text).

https://doi.org/10.1371/journal.pone.0051267.s003

(DOC)

Acknowledgments

We thank Elwira Szuma for her assistance at the Mammal Research Institute collection. Two anonymous reviewers provided useful comments on an earlier draft of this manuscript.

Author Contributions

Conceived and designed the experiments: NS ACA JAD. Performed the experiments: NS KAH ACA AZ JAD. Analyzed the data: KAH NS. Contributed reagents/materials/analysis tools: NS KAH ACA AZ JAD. Wrote the paper: NS KAH AZ JAD. Critical review of the manuscript: NS KAH ACA AZ JAD.

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