Observations of Immuno-Gold Conjugates on Influenza Viruses Using Waveguide-Mode Sensors
Figure 7
Analyses of pre-mixed A/Udorn/307/1972 with immuno-AuNPs on captured anti-rabbit IgG.
(a) Waveguide spectrum of A/Udorn/307/1972 that was pre-mixed with immuno-AuNPs; Viruses from 8×102 PFU/ml to 8×105 PFU/ml were titrated. 10% of the immuno-AuNPs were used. Vertical reflectivity changes are considered. Gold nanodots forming a confined ring on the surface of the virus are shown as figure inset. The blue and brown lines represent the reflectivity measured after the attachment of ethanolamine and immuno-AuNP-virus complex, respectively, on the CDI surface immobilized with anti-rabbit IgG as the capture antibody. (b) Graphical representation of spectral changes with the above interactions. Error bars are shown with averaged values. Values less than 3% was considered to be within the error ranges and the minimum detectable limit is 8×103 PFU/ml of A/Udorn/307/1972.