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Quantitative Evaluation of the Mitochondrial Proteomes of Drosophila melanogaster Adapted to Extreme Oxygen Conditions

Figure 6

The differential mitochondrial proteins derived from iTRAQ were confirmed by the MRM analysis.

The target proteins were selected base on the principles described in the text. The numbers 1–10 in the X axis represent targets as FBpp0071128, FBpp0074481, FBpp0088174, FBpp0078847, FBpp0077425, FBpp0077859, FBpp0070370, FBpp0072231, FBpp0087333 and FBpp0073949 (all from mitochondrial respiratory chain complex I), respectively. The Y axis indicates the abundance ratios of the individual targets that are normalized by the corresponding abundance of control. All the samples came from two biological replicates, and each sample was analyzed by MRM with triplicate. The calculation of abundance averages with standard deviations and the t-test were conducted with Excel (Microsoft Office 2010, USA). Black: HOF; Light gray: Control; Dark gray: LOF. All the abundances of components in HOF were significantly lower (* p<0.05, n = 6) than that of control and LOF.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0074011.g006