Structures of Wnt-Antagonist ZNRF3 and Its Complex with R-Spondin 1 and Implications for Signaling
Figure 4
ZNRF3-RSPO1 binding site coincides with LGR5-RSPO1 ‘trans’ site.
Shown in cartoon with transparent surface representation are the crystal structure of the 2:2 LGR5-RSPO1 complex (PDB code 4BSR) and the structure of ZNRF3-RSPO1 superimposed on RSPO1 on the right-hand side (orange), only the Cα trace of ZNRF3 (red) is shown for clarity. The overlapping LGR5 chain is shown in blue. The remaining part of the 2:2 LGR5-RSPO1, i.e. left-hand side RSPO1 and right-hand side LGR5, is shown in grey.