Deficient EBV-Specific B- and T-Cell Response in Patients with Chronic Fatigue Syndrome
Figure 6
Latent EBV can be detected more frequently in CFS patients.
(A) EBV DNA was analyzed via nested real-time PCR in total PBMCs of 20 healthy donors and CFS patients for EBER-1. EBER-copies were calculated in accordance to Namalwa standard. (B) BZLF-1 RNA was analyzed via nested real-time PCR in total PBMCs of 20 healthy donors and CFS patients but no BZLF-1 cDNA was detected. cDNA of EBV cell line 293T/B95-8 was used as positive control. Statistical analysis was performed using the one-tailed Mann-Whitney-U test with ** p<0.01.