Application of a Genetically Encoded Biosensor for Live Cell Imaging of L-Valine Production in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains
Figure 5
Biosensor-driven analysis of phenotypic heterogeneity.
In the presence of low amounts of complex carbon sources, significant cell-to-cell variability in the switch from growth to L-valine production was observed. (A) Growth and production phase (initiated after 340 min) of an isogenic microcolony of the ΔaceE sensor strain and (B) the lineage tree of the respective microcolony highlighting several single cell traces. EYFP fluorescence was quantified in single cells after 860 min. (C) Single cell traces of fluorescence output of marked cells (see A and B) and average emission of the whole colony (black, dashed line, SD = grey shading). Cultivation was performed in CGXII minimal medium containing 154 mM acetate, 222 mM glucose and 0.5% BHI during growth phase or 222 mM glucose and 0.5% BHI during production phase, respectively.