Silencing Motifs in the Clr2 Protein from Fission Yeast, Schizosaccharomyces pombe
Figure 7
Point mutations in Clr2 affected transcriptional silencing at several locations in the S. pombe genome.
Cells were diluted by a factor of 5 for each and spotted on +URA, selective –URA and counter-selective FOA plates. Top lane is the wild type strain (Clr2) that grows poorly on the –URA plates. Second lane is a strain lacking Clr2 (Δ), third contains the V5-Clr2 followed by the point mutations in Clr2; Clr2P137G, Clr2Y140G, Clr2L142G, Clr2R170G, Clr2H178G, Clr2L182G and Clr2E376G. The reporter gene is (A) rDNA::ura4+ (B) cc2(SphI)::ura4+ and in (C) the ade6+ reporter gene inserted into the ura4 locus and with Gal4DBD-Clr4ΔCD resulting in repression of the ade6+gene.