Oncogenic H-Ras Up-Regulates Acid β-Hexosaminidase by a Mechanism Dependent on the Autophagy Regulator TFEB
Figure 8
Immunoblotting analysis of TFEB expression in HuDe fibroblasts transfected with H-Ras mutants.
A, Cytoplasm extracts were tested with anti-H-Ras antibody. As internal control an anti-β actin antibody was used. Nuclear extracts were tested with an anti-TFEB antibody. As internal control, an anti-H3 histone antibody was used. B, Model of lysosomal HEXA gene regulation by TFEB through a Raf/ERK dependent pathway.