Desmoglein 2 Depletion Leads to Increased Migration and Upregulation of the Chemoattractant Secretoneurin in Melanoma Cells
Figure 8
Immunohistochemistry of SgII and Dsg2 on paraffin sections of primary malignant melanomas and melanoma metastases.
(A-C, J-L) Primary nodular malignant melanoma (NMM) with a tumor thickness of 0.8 mm (pT1a; no. 4 in the Table S3). (D-F, M-O) Primary NMM of 10 mm thickness (pT4a; no. 1). (G-I) Melanoma metastasis of the mamma (no. 8). (P-R) Cutaneous melanoma metastasis of the temple (no. 15). In the upper part of the figure (A-I), tumors were immunostained with two different SgII antisera (A, D, G: GTX116446 and B, E, H: LS-C39034), or, for control, with secondary goat anti-rabbit HRP-IgG (C, F, I). Clearly positive immunoreactions are seen in the cytoplasm of the melanoma cells with both SgII antisera in all tumors examined. In the lower part (J-R), tumors were reacted with antibodies against Dsg2 (rb5). Diffuse Dsg2-positive reactions are detected in the cytoplasm and/or at the surface of the melanoma cells (J, M, P: overviews; K, N, Q: higher magnifications). In addition, Dsg2 appears to be focally enhanced at the cell-cell contacts of the primary NMM of 10 mm thickness and the cutaneous metastasis (N, Q). Dsg2-positive structures serving as internal positive controls on each slide, i.e. sweat glands (L), basal epidermis (O) and hair follicles (R) are shown on the right hand side of each row. Bars: 50 µm (L, Q), 200 µm (P) or 100 µm (all other micrographs).