pSiM24 Is a Novel Versatile Gene Expression Vector for Transient Assays As Well As Stable Expression of Foreign Genes in Plants
Figure 14
Transient expression of nat-T-phyllo-GFP using pSiM24 expression vector.
(A) RT-PCR products for native T-phylloplanin fused GFP (nat-T-phyllo-GFP) with the expected 1304 bp band are shown. Transiently nat-T-phyllo-GFP expressed agro-infiltrated N. tabacum L. variety Samsun NN leaf samples using pSiM24-nat-T-phyllo-GFP (P1, P2, P3 and P4) and pSiM24 (Vc; empty vector control) are presented. (B) Localization analysis of apoplast targeted nat-T-phyllo-GFP by confocal laser scanning microscopy. Agro-infiltrated N. tabacum L. variety Samsun NN leaf cells for pSiM24-nat-T-phyllo-GFP construct expressing GFP (green fluorescence) was visualized by confocal laser scanning microscopy. No GFP fluorescence was detected in agro-infiltrated tobacco leaf cells for pSiM24 construct (Control). Scale bar represents 20 µm on all images. Fluorescent, bright-field and superimposed (bright-field and green fluorescent) confocal laser scanning micrographs of leaf sections are presented.