Light Scattering Sensor for Direct Identification of Colonies of Escherichia coli Serogroups O26, O45, O103, O111, O121, O145 and O157
Figure 6
Light-scattering analysis and PCR confirmation of colonies of serogroup O157:H7 (EDL933) and O26:H11 (90-0105) on SMAC agar.
(A) Scatter images of colonies of O26, O157 and the mixture of O26 and O157 grown on SMAC at 37°C for about 11 h. Representative colonies that were analyzed by multiplex PCR (mPCR) are labeled as C1-C15. (B). Agarose gels showing PCR amplified bands for each colony tested.