Th1 Stimulatory Proteins of Leishmania donovani: Comparative Cellular and Protective Responses of rTriose Phosphate Isomerase, rProtein Disulfide Isomerase and rElongation Factor-2 in Combination with rHSP70 against Visceral Leishmaniasis
Figure 10
Antibody response in vaccinated hamsters.
IgG (A and D) and its isotypes IgG1 (B and E) and IgG2 (C and F) in vaccinated hamsters in comparison to the non-vaccinated infected hamsters on days 45 and 90 p.c. Serum samples were collected from different experimental groups of hamsters at designated time points and assayed for specific IgG, IgG1, and IgG2 levels by ELISA. Data are presented as the absorbance at 492 nm and are means ± SD for 3–4 hamsters per group in triplicate wells. Data are representative of two independent experiments with similar results. Significance values indicate the difference between the vaccinated and infected group (*, p<0.05; **, p<0.01; and ***, p<0.001).