Direct Detection and Differentiation of Pathogenic Leptospira Species Using a Multi-Gene Targeted Real Time PCR Approach
Figure 2
Illustration of the real-time PCR amplification curves obtained during the testing of naturally-infected tissue samples.
(A) Results of the β-actin and lipL32 targeted duplex amplification assay when testing representative samples from the wild rodents. The partial β-actin gene was amplified from all tissue samples (dark pink lines). Leptospiral DNA was detected in three samples by a positive amplification of the lipL32 gene (blue lines). A spiked positive control with L. interrogans (serovar Autumnalis, strain Akiyami) is shown (green line). (B) From the previous leptospiral positive amplification results, two samples were assessed as infected with L. borgpetersenii using the respective targeted amplification assay with probe TqM_bpn and flanking primers F_bpn and R_bpn1 (blue lines). The positive and negative controls are illustrated by the orange and red lines, respectively.