Cell culture and transfection

Human embryonic kidney cells (HEK293) (ATCC) were cultured under standard condition (5% CO₂, 37°C) in High Glucose Dulbecco's modified Eagle's medium (DMEM) (Sigma Chemicals) containing 10% fetal bovine serum with 100 µg/ml penicillin-streptomycin. The CaSR mutations were introduced using site-directed mutagenesis (QuikChange, Stratagene, La Jolla, CA, USA) [14]. Cells were transfected with either pEGFP-N1-WT-CaSR or mutant CaSRs using Lipofectamine 2000 in reduced serum Opti-MEM medium following the manufacturer's instructions (Invitrogen). After 4∼6 hours, the medium was changed to High Glucose DMEM, and the cells were incubated for an additional 48 hours to increase receptor expression. Transfection efficiency and expression levels were confirmed by analyzing the fluorescence intensity of the EGFP-tagged CaSR by fluorescence microscopy (Leica, DMI600B).

Measurement of [Ca²⁺]_i responses in single cells transfected with WT or mutant CaSRs

Measurement of [Ca²⁺]_i was carried out as described by Jiang, et al. [15]. Briefly, wild type CaSR or its mutants were transiently transfected into HEK293 cells grown on coverslips and cultured for 48 h. The cells were subsequently loaded with Fura-2 by incubation with 4 µM Fura-2 AM in 2 mL physiological saline buffer (10 mM HEPES, 140 mM NaCl, 5 mM KCl, 1.0 mM MgCl₂, 1 mM CaCl₂ and pH 7.4). The coverslips were mounted in a bath chamber on the stage of a Leica DM6000 fluorescence microscope. The cells were alternately illuminated with 340 or 380 nm light, and the fluorescence at an emission wavelength of 510 nm was recorded in real time as the concentration of [Ca²⁺]_o was increased in a stepwise manner in the presence or absence of 5 mM L-Phe. The ratio of emission fluorescence intensity from both excitation wavelengths was then monitored as a function of [Ca²⁺]_o and utilized as a parameter reflecting changes in [Ca²⁺]_i All experiments were performed at room temperature. In addition to the use of the cell population assay as a means of calculating EC₅₀ for Ca²⁺_o or L-Phe, the signals from 30 to 60 single cells were recorded for each measurement in a single cell [Ca²⁺]_i assay. In the latter assay, [Ca²⁺]_i oscillations in individual cells were defined as three or more successive fluctuations in [Ca²⁺]_i from the baseline after the initial peak.

Immunostaining and Western blotting of CaSR

pcDNA3.1-CaSRs were used in the immunostaining and Western blotting experiments. This CaSR construct contains a flag-tag between Asp³⁷¹ and Thr³⁷². For immunostaining, 48 hours after transfection, cells were fixed with 3.7% formaldehyde for 15 min at room temperature, and subsequently labeled with mouse anti-Flag monoclonal antibody (1∶3000) overnight at 4°C. The next day cells were washed with PBS and stained with goat anti-mouse Alexa488-conjugated secondary antibodies. Images were collected on a Zeiss LSM700 confocal microscope (Carl Zeiss, German). For Western blotting, cells were lysed with RIPA buffer (Millipore, MA). Protein concentrations were determined using a Bradford assay (Bio-Rad laboratories, Hercules, CA). Lysates containing 40 µg of protein were separated by 7.5% SDS-PAGE. After gel electrophoresis, the proteins were transferred to a nitrocellulose membrane, probed with anti-flag monoclonal antibody (1∶3000) in 3% milk/TBST overnight at 4°C. Blots were subsequently probed with alkaline phosphatase-conjugated secondary antibody (1∶3000) for 1 hour at room temperature. After washing, the signals were detected by standard enhanced chemiluminescence. The signals were quantitated using ImageJ.

Measurement of inositol-1-phosphate (IP₁) accumulation in CaSR transfected HEK293 cells

HEK293 cells were seeded in 24-well plates at 3×10⁵ cells per well in 500 µl of culture medium. After transfection with WT CaSR or its various mutants, cells were further cultured for 24 hours at 37°C. Cell monolayers were first washed with Ringer's buffer without calcium (121 mM NaCl, 2.4 mM K₂HPO₄, 0.4 mM KH₂PO₄, 10 mM HEPES, 5.5 mM glucose, 1.2 mM MgCl₂) and then incubated for 1 hour at 37°C in stimulation buffer (140 mM NaCl, 5 mM KCl, 10 mM LiCl₂, 0.55 mM MgCl₂, 10 mM HEPES) containing varying concentrations of CaCl₂. After treatment, cells were lysed for 30 min at 37°C with 50 µl of 2.5% IP₁ ELISA Kit Lysis Reagent (CIS Bio International, Gif-sur-Yvette, France). The accumulation of IP₁ was measured using an immunoassay based on competition between free IP₁ and horseradish peroxidase (HRP) conjugated IP₁ for binding to monoclonal anti-IP₁ antibody. The results for IP₁ were expressed as percentage inhibition of IP₁-HRP binding  =  [1-IP₁-HRP binding in stimulated cells/IP₁-HRP binding in unstimulated cells] ×100. The EC₅₀s of [Ca²⁺]_o-dependent responses were calculated by fitting the [Ca²⁺]_o concentration-response curves with the Hill equation.

Determination of ERK_1/2 phosphorylation

Thirty-six hours post transfection of monolayers of HEK293 cells with CaSR or its mutants, cells were incubated in serum-free high glucose DMEM medium supplemented with 0.2% w/v BSA at 37°C overnight. On the following day, cells were first incubated with HBSS for 30 min, followed by stimulation with varying levels of CaCl₂ (0–20 mM) with or without L-Phe (5 mM) for 10 min. At the end of the [Ca²⁺]_o stimulation, cells were lysed with RIPA lysis buffer (Millipore, CA, USA). In total, 150 µg aliquots of lysate protein were loaded into either a 12.5% SDS-gel or a 4%–12.5% gradient gel for PAGE and analyzed by western blotting with an anti-phospho-p44/42 ERK polyclonal antibody (Cell Signaling Technology, Beverly, MA, USA) diluted (1∶2000). A chemiluminescent method (AP Conjugate Substrate Kit) was employed to detect the phospho-(p)44/42 proteins. Quantitative analysis of the results was performed using ImageJ software (National Institutes of Health). The responses were normalized to the maximal effect observed with [Ca²⁺]_o alone. The EC₅₀ of [Ca²⁺]_o-dependent responses was calculated by fitting the [Ca²⁺]_o concentration-response curves with the Hill equation.

Computational analysis and Molecular Dynamics (MD) simulation of CaSR

CLUSTALW was used to align the sequences of the human CaSR ECD (residues 25–530) and the mGluR1 ECD [16]. The structure of the ECD of CaSR was modeled based on the crystal structure of mGluR1 (1EWT, 1EWK and 1ISR) using SWISS-MODEL [17] and MODELLER software [18], and the potential Ca²⁺-binding sites in the CaSR ECD were predicted using MetalFinder [19], The electrostatic potentials were calculated using Pymol.

MD simulation provides an approach complementary to experiments in live cells for understanding biomolecular structure, dynamics, and function. The initial coordinates for all the simulations were taken from a 2.20 Å resolution x-ray crystal structure with PDBID 1EWK [20]. The AMBER 10 suite of programs [21] was used to carry out all of the simulations in an explicit TIP3P water model [22], using the modified version of the all-atom Cornell et. al. [23] force field and the re-optimized dihedral parameters for the peptide ω-bond [24]. An initial 2 ns simulation was performed using NOE restraint during the equilibration in order to reorient the side chains of residues in the Ca²⁺-binding site, but no restraints were used during the actual simulation. A total of three MD simulations were carried out for 50 ns each on the apo-form and the ligand-loaded forms. During the simulations, an integration time step of 0.002 ps was used to solve the Newton's equation of motion. The long-range electrostatic interactions were calculated using the Particle Mesh Ewald method, [25], and a cutoff of 9.0 Å was applied for non-bonded interactions. All bonds involving hydrogen atoms were restrained using the SHAKE algorithm [26]. The simulations were carried out at a temperature of 300 K and a pressure of 1 bar. A Langevin thermostat was used to regulate the temperature with a collision frequency of 1.0 ps-1. The trajectories were saved every 500 steps (1ps). The trajectories were analyzed using the ptraj module in AMBER 10.

Statistics

The data are presented as means ± SE of the indicated number of experiments. One-way repeated measures analysis of variance (ANOVA), followed by the Dunnett post hoc test was performed using SPSS (SPSS Inc, Chicago, IL, USA) for multiple comparisons to compare the differences between groups without adding L-Phe. Two-way ANOVA was used for comparison among different groups in the presence of L-Phe. A P value of < 0.05 was considered to indicate a statistically significant difference.

The disease-associated CaSR mutations alter the [Ca²⁺]_o-triggered [Ca²⁺]_i oscillation pattern

The two residues (Leu¹⁷³, Pro²²¹) involved in the four disease-associated mutations studied here are located in the hinge region near the predicted calcium-binding site 1, which is located between lobe 1 and lobe 2. Meanwhile they are also adjacent (within 10 Å) to the reported L-Phe binding pocket formed by residues K47, L51, W70, T145, G146, S169, S170, I187, Y218, S272, H413 and R415, which can exert effects over a large region of the ECD through positive heterotropic cooperativity as shown in Figure 1a [12]. The immunostaining as well as the Western blotting results suggest that all of the CaSR mutants are expressed at the cell surface at similar levels (Figure 1b, 1d, 1e).

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Figure 1. The disease-related mutations are located in the hinge region near Ca²⁺-binding site 1.

a. Model structure of CaSR ECD based on the mGluR1 crystal structure (PDB entry: 1ISR) was generated using MODELER 9v4 and Swiss-Model. The global view of the ECD is shown in the left panel. Spheres highlighted in red: Ca²⁺; Purple: residues involved in predicted Ca²⁺-binding sites. L-phenylalanine (in yellow) is positioned at the hinge region between the two lobes by Autodock vina. Right panel: A zoomed in view of the Ca²⁺-binding pocket of site 1. Residues involved in Ca²⁺-binding site 1are highlighted in purple; residues with disease-related mutations are highlighted in pink; residues predicted to interact with L-Phe are presented in green. b. Immunofluorescence analysis of surface expressed WT CaSR and its mutants in HEK293 cells. Immunostaining was done with anti-flag monoclonal antibody, and detection was carried out with Alex Fluor 488-conjugated, goat anti-mouse secondary antibody. Green: CaSR. The images were taken using equal exposure times. c. Schematic figure of calcium and L-Phe induced downstream signaling changes and the principle for the measurement of IP₁ accumulation. Red dots represent calcium ions. d. total proteins (40 µg) were applied to SDS-PAGE under non-reducing conditions and blotted with anti-flag antibody. GAPDH was used as an internal control. e. The signals were analyzed using ImageJ and the normalized intensities were compared with WT CaSR.

https://doi.org/10.1371/journal.pone.0113622.g001

Analysis of the [Ca²⁺]_o-triggered [Ca²⁺]_i signaling (Figure 1c), especially the pattern of [Ca²⁺]_i oscillations, is a straightforward method to depict the potential alterations in the homotropic cooperativity induced by mutations. Three parameters were employed to analyze the [Ca²⁺]_i oscillation patterns: the starting point, which refers to the [Ca²⁺]_o at which a given cell starts to show at least three continuous, sequential peaks; the frequency, which is defined as the number of peaks of [Ca²⁺]_i per minute, and the ending point, which is the [Ca²⁺]_o at which the [Ca²⁺]_i ceases to oscillate and reaches a plateau. About 53% of the cells transfected with WT CaSR showing oscillatory responses started to oscillate at 3.0 mM [Ca²⁺]_o (Figure 2 & 3). However, the majority of cells transfected with the inactive mutant, P221Q, did not oscillate until [Ca²⁺]_o reached more than 4.0 mM, and the threshold for [Ca²⁺]_i oscillations in cells transfected with the other inactive mutant, L173P, was even higher at 12.5 mM. Conversely, for the gain-of-function mutations (L173F and P221L), the majority of the cells, 79% for L173F and 40% for P221L, started to oscillate at lower levels of [Ca²⁺]_o, 2.0 mM and 2.5 mM, respectively.

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Figure 2. Functional studies of disease-related CaSR mutations in individual HEK293 cells.

The various panels show representative oscillation patterns from single cells. HEK-293 cells transfected with CaSR or one of its mutants were loaded with Fura-2 AM for 15 min. [Ca²⁺]_i was assessed by monitoring emission at 510 nm with excitation alternately at 340 or 380 nm as described in Methods. Each experiment was carried out with or without 5 mM L-Phe and began in Ca²⁺-free Ringer's buffer (10 mM HEPES, 140 mM NaCl, 5 mM KCl, and 1.0 mM MgCl₂, pH 7.4), followed by stepwise increases in [Ca²⁺]_o until [Ca²⁺]_i reached a plateau (up to 30 mM [Ca²⁺]_o).

https://doi.org/10.1371/journal.pone.0113622.g002

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Figure 3. Frequency distribution of oscillation parameters in HEK293 cells transfected with CaSR or its mutants.

The pattern of the [Ca²⁺]_i response in each cell (minimum of 40 cells) was analyzed. a. The [Ca²⁺]_o at which individual cells started to oscillate was recorded. The X-axis comprises 1.0 mM to 8.0 mM [Ca²⁺]_o for WT and for mutants L173F and P221L, 1.5 mM to 10.0 mM [Ca²⁺]_o for P221Q or 7.5 mM to 25.0 mM for mutant L173P. b. The frequency of the individual cell oscillation patterns was investigated. For experiments without L-Phe, the peaks per minute were recorded at the levels of [Ca²⁺]_o at which the majority of the cells (>50%) started oscillating. Specifically, for the gain-of-function mutants, the peaks per minute were recorded at 2.5 mM [Ca²⁺]_o, while for loss-of-function mutants, the frequency was analyzed at 15.0 mM [Ca²⁺]_o for L173P and 5.0 mM [Ca²⁺]_o for P221Q. c. The [Ca²⁺]_o at which the [Ca²⁺]_i oscillations began to reach a plateau was recorded. Empty bar: in the absence of L-Phe; Black bar: in the presence of 5 mM L-Phe.

https://doi.org/10.1371/journal.pone.0113622.g003

WT CaSR-transfected cells exhibited a normal distribution of oscillation frequencies with an average frequency of 1.0∼1.5 peaks/min at 3.0 mM [Ca²⁺]_o (Figs. 2&3b, Table 1). The two inactivating mutants (L173P and P221Q) responded to changes in [Ca²⁺]_o over a substantially different range. Therefore, the frequencies were measured at the level of [Ca²⁺]_o at which the majority of the cells (>50%) started to oscillate—15 mM for L173P and 5 mM for P221Q. For L173F and P221L, the frequencies were measured at 2.5 mM [Ca²⁺]_o. The oscillation frequency for the two inactivating mutants did not differ from that of the WT (1.3±0.1 peaks/min) and were 1.3±0.1 peaks/min for L173P and 1.5±0.1 peaks/min for P221Q, but there were slight but significant increases for L173F (1.7±0.1 peaks/min) and P221L (1.6±0.1 peaks/min).

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Table 1. Summary of cellular responses of HEK293 cells transiently transfected with WT CaSR or disease related mutants.

https://doi.org/10.1371/journal.pone.0113622.t001

We also studied the oscillation ending point. About 50% of the oscillatory cells expressing the CaSR reached a [Ca²⁺]_i plateau (i.e., stopped oscillating) at 5.0 mM [Ca²⁺]_o (Figure 3c). The loss-of-function mutations exhibited a dramatic increase in the [Ca²⁺]_o required for a [Ca²⁺]_i plateau as shown in Figure 3c. The majority of the oscillatory cells transfected with L173P were still exhibiting [Ca²⁺]_i fluctuations even at a level of [Ca²⁺]_o of 30.0 mM. Similarly, the ending point for P221Q was substantially elevated at ∼10.0–15.0 mM. On the other hand, quite a few cells transfected with either of the gain-of-function mutants stopped [Ca²⁺]_i oscillations at a [Ca²⁺]_o below 5.0 mM. Therefore, the [Ca²⁺]_o-triggered [Ca²⁺]_i oscillation pattern, including its frequency, the [Ca²⁺]_o required for initiating oscillations, and the oscillation ending point are altered in the four mutations, suggesting changes in [Ca²⁺]_o–elicited positive homotropic cooperativity induced by these mutations.

The disease-associated CaSR mutations affect functional positive homotropic cooperativity as reflected by changes in multiple CaSR-activated signaling pathways.

We then investigated how those mutations affect the CaSR-mediated activation of various intracellular signaling pathways. HEK293 cells transfected with WT CaSR exhibited sigmoidal concentration response curves for the [Ca²⁺]_i responses to increases in [Ca²⁺]_o in a cell population assay with a Hill coefficient of 3.7±0.3 and an EC₅₀ of 3.0±0.2 mM, suggesting strong positive homotropic cooperativity among its five predicted Ca²⁺-binding sites (Figure 4, Table 1). Notably, the two inactivating mutations showed increases in EC₅₀ and disruption of the CaSR's positive homotropic cooperativity, with a change in the monophasic [Ca²⁺]_o-induced [Ca²⁺]_i response curves to a biphasic one (L173P) or a decrease in Hill number for P221Q, accompanied by an increase of EC₅₀ to 5.2±0.4 mM (Figure 4, Table 1).

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Figure 4. L-Phe modulates the [Ca²⁺]_o concentration response curves in CaSR-transfected HEK293 cells.

The [Ca²⁺]_i responses of HEK293 cells transiently overexpressing WT CaSR or disease-related mutations were measured using Fura-2AM during stepwise increases in [Ca²⁺]_o from 0.5 to 30 mM with or without L-Phe as above. The ratio of light emitted at 510 nm upon excitation alternately with 340 or 380 nm was normalized to the maximum response. And the average [Ca²⁺]_i responses at various [Ca²⁺]_o were normalized and plotted against [Ca²⁺]_o and further fitted using the Hill equation. Open marker: in the absence of L-Phe; Closed marker: in the presence of 5 mM L-Phe.

https://doi.org/10.1371/journal.pone.0113622.g004

Activation of the G_q/11 pathway by GPCRs activates phospholipase C (PLC), which induces an increase in [Ca²⁺]_i caused by the associated accumulation of inositol phosphates (IPs) [3]. Thus the activation of PLC in response to increases in [Ca²⁺]_o in cells transiently transfected with WT CaSR were assessed by measuring the accumulation of IP₁ as an index of PLC activation Figure 1c & Figure 5). The EC₅₀ for the stimulation of IP₁ accumulation was 2.9±0.3 mM, similar to the EC₅₀ calculated from the [Ca²⁺]_i responses (3.0±0.2). As with their Ca²⁺_i responses, L173P and P221Q exhibited impaired [Ca²⁺]_o–stimulated IP₁ accumulation as reflected by the increases in their EC₅₀s, 10.7±1.0 mM and 6.1±0.3 mM respectively, vs. 2.9±0.3 mM for the WT) (Figure 5, Table 2). In contrast, the L173F and P221L mutants showed substantial decreases in their EC₅₀s to 1.2±0.1 mM and 1.1±0.1 mM, respectively (Table 2).

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Figure 5. L-Phenylalanine potentiates [Ca²⁺]_o-induced IP₁ accumulation.

IP₁ accumulation was measured using the IP-One ELISA Kit as detailed in Methods. Left: Changes in IP₁ accumulation were measured in response to incubation with 0, 1.0, 2.0, 3.0, 5.0 and 7.5 mM [Ca²⁺]_o in HEK293 cells transfected with WT CaSR, or with mutants L173F or P221L. For mutant L173P, IP₁ accumulation was measured in response to 0, 3.0, 5.0, 7.5, 10.0, 15.0, 20.0 and 25.0 mM [Ca²⁺]_o; for mutant P221Q, the IP₁ responses at 0, 3.0, 5.0, 7.5, 10.0, 15.0 mM [Ca²⁺]_o were recorded. The IP₁ response-calcium concentration responses were fitted using Hill equation as described in Methods.

https://doi.org/10.1371/journal.pone.0113622.g005

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Table 2. Summary of EC₅₀s from experiments measuring IP₁ accumulation and ERK_1/2 phosphorylation.

https://doi.org/10.1371/journal.pone.0113622.t002

We further examined the effects of [Ca²⁺]_o on mitogen-activated protein kinases (MAPK) (ERK_1/2) activation. Exposure of WT CaSR-transfected HEK293 cells to increasing concentrations of [Ca²⁺]_o in the range of 0.0-∼2.0 mM for 10 minutes had little effect on the phosphorylation of p44/42 ERK. Greater increases of [Ca²⁺]_o resulted in the accumulation of p44/42 ERK, which exhibited a maximum response at 8.0 mM [Ca²⁺]_o. Immunoblots of [Ca²⁺]_o–induced increases in ERK activity were further quantified using ImageJ and plotted against [Ca²⁺]_o. The data were then fitted using the Hill equation, giving an EC₅₀ of around 3.0±0.1 mM, which is close to the result measured using intracellular calcium responses. The mutants L173P and P221Q also impaired the [Ca²⁺]_o-triggered ERK activity of the CaSR. More than 8.0 mM [Ca²⁺]_o was needed in order to induce the phosphorylation of ERK_1/2 for both mutants (Figure 6). For the activating mutation, L173F, a phosphorylated ERK_1/2 signal could be detected at 2.0 mM [Ca²⁺]_o, which was in line with its enhanced intracellular calcium response compared to WT CaSR. The mutant P221L also potentiated [Ca²⁺]_o-evoked ERK_1/2 activity in the transfected cells, approaching a maximum at 3.0 mM [Ca²⁺]_o.

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Figure 6. L-Phe potentiates [Ca²⁺]_o-activated ERK signaling in CaSR-transfected HEK293 cells.

HEK-293 cells transfected with WT CaSR or its mutants were incubated in serum-free high glucose MEM medium containing 0.2% BSA overnight. Cells were washed with Hank's balance salt solution (HBSS) and then incubated in the presence of various Ca²⁺ concentrations (0.0-∼25.0mM) in the absence or presence of 5 mM L-phenylalanine for 10 min at 37°C. The incubations were stopped by exposure to the lysis buffer and processed for SDS/PAGE and Western blotting as described in the Methods. The Western blot results were further quantified using Image J. All [Ca²⁺]_o-concentration response curves were normalized to the maximum response in each individual experiment. The Hill equation was employed to fit the data. Markers: [Ca²⁺]_o only; closed markers: with L-Phe.

https://doi.org/10.1371/journal.pone.0113622.g006

CaSR mutants exhibit different sensitivities to L-Phe.

To determine whether or not the distinctive effects shown by L-Phe on different mutants are due to changes in their apparent affinities for this amino acid, concentration responses for L-Phe at a physiological level of 1.5 mM [Ca²⁺]_o were first compared among cells transfected with different CaSR mutants. Figure 7 shows that at 1.5 mM [Ca²⁺]_o, the loss-of-function mutant L173P exhibited a lower sensitivity (EC₅₀ = 5.5±0.3 mM) to L-Phe compared to WT and the other mutants (all EC₅₀s<5.0 mM). Since the influence of L-Phe might, to some extent, depend on the extracellular calcium concentration, the apparent affinities of L-Phe for the two loss-of-function mutants were further analyzed at levels of [Ca²⁺]_o equal to their respective EC₅₀s for [Ca²⁺]_o, specifically 15.0 mM [Ca²⁺]_o for L173P and 5.0 mM [Ca²⁺]_o for P221Q. P221Q exhibited a reduced EC₅₀ for the L-Phe response (EC₅₀ = 2.5±0.2 mM, p<0.05) at 5.0 mM [Ca²⁺]_o compared with EC₅₀ for L-Phe measured at 1.5 mM [Ca²⁺]_o (EC₅₀ = 4.5±0.4 mM), while L173P retained a similar sensitivity to L-Phe regardless of the extracellular calcium concentration (EC₅₀ = 4.9±0.5 mM, p>0.05) (Figure 7, Table 3).

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Figure 7. Sensitivity of various CaSR mutants to L-Phe in HEK293 cells.

a. HEK-293 cells transfected with CaSR or its mutants were loaded with Fura-2 AM for 15 min. The intracellular Ca²⁺ level was assessed by monitoring emission at 510 nm with excitation alternately at 340 or 380 nm using fluorescence microscopy as above. Each experiment started with 1.5 mM mM Ca²⁺ followed by stepwise increases in the level of L-Phe (up to 12.0 mM) while the [Ca²⁺]_o was maintained at 1.5 mM. b. The sensitivity of P221Q and L173P to L-Phe were compared at 5.0 mM [Ca²⁺]_o or 15.0 mM [Ca²⁺]_o, respectively.

https://doi.org/10.1371/journal.pone.0113622.g007

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Table 3. Summary of EC₅₀s of concentration-response curves for L-Phe at different [Ca²⁺]_o.

https://doi.org/10.1371/journal.pone.0113622.t003

Computational simulations reveal different dynamic behaviors of the four mutants.

The correlated motions in Figure 8 illustrate the molecular dynamic motions of WT CaSR and its mutants obtained from computational simulations. In the modeled WT structure, Site 1 has strong correlated motions with sites 2, 3 and 4, shown in blue (negative correlated motions between a pair of residues signify motions in opposite direction) and red (positive correlations, which are movements in the same direction) (Figure 8, top two panels). We also have reported in our earlier work that the presence of both L-Phe and Ca²⁺ in site 1 produces greater correlated motions among the Ca²⁺-binding sites compared with L-Phe alone in site 1 [12]. Interestingly, the loss-of-function mutations, L173P and P221Q, exhibit less correlated motions compared to the WT CaSR as demonstrated by a decrease in the negative (blue) and/or positive correlated motions (Figure 8, bottom panel). On the other hand, the correlated motions of the gain-of-function mutants, L173F and P221L, are dramatically increased in regions similar to those of the WT correlated motions in the presence of L-Phe, indicating an enhancement of correlated motions between the respective residues in these two mutants (Figure 8, middle panel). A closer analysis of the correlated motions of L173F and P221L reveals that the negative correlations between Site 1 and Site 3 and between Site 1 and Site 2 observed in the WT correlation map become positive correlations in the gain-of-function mutants indicating that the mutations might have profound impacts on the dynamic properties of the CaSR-ECD. Especially for P221L, strong negative correlations between lobe 1 and lobe 2 are observed as indicated by the abundant blue area between residues 200–300 and residues 24–170. These results indicate that residues Leu¹⁷³ and Pro²²¹ play pivotal roles in modulating the molecular connectivity between the binding sites for [Ca²⁺]_o. In order to examine the possibility that the effects of inactivating mutations of the CaSR can be cancelled by those of activating mutations through molecular correlated motions, two double mutations, L173F/P221Q and L173P/P221L have been analyzed. We found that L173F/P221Q behaved like WT CaSR, thereby exhibiting a “cancelling” effect (Figure S1a), while L173P/P221L exhibited oscillation patterns similar to those of the L173P mutant, suggesting that the activating mutant P221L cannot overcome the inactivating effect of L173P (Figure S1b). These results suggest that mutations close to calcium binding Site 1 may play key roles in modulating correlated motions of the CaSR.

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Figure 8. Correlation map of the modeled WT and mutant CaSR ECD structures.

The correlation maps are depicted based on molecular dynamic (MD) simulations of the WT and mutant CaSR ECD structures in the apo form or in the presence of Ca²⁺ and L-Phe. The X axis and Y axis are residue numbers of the CaSR ECD sequence minus twenty four. The strongest negative correlation is given the value -1, while the strongest positive correlation is defined as +1. Residues that have the strongest negative correlated motions are shown in blue, while those involved in positive correlated motions are shown in red. Green indicates no apparent correlated motion between the two residues. The correlated motions between calcium binding site 1 and the other calcium binding sites are mapped onto the CaSR ECD models as shown in the top right panel.

https://doi.org/10.1371/journal.pone.0113622.g008

The docking of L-Phe to CaSR mutant P221Q engendered a more correlated molecular dynamics features similar to WT CaSR ECD, suggesting that the receptor is converted into an active conformation. Intriguingly, the other inactive mutant L173P did not exhibited dramatic alterations in its correlated motion in response to L-Phe compared to P221Q. This result suggests that the difference in the influence of L-Phe on potentiating the activity of the CaSR mutants may rely on the intrinsic molecular dynamic connectivity that is disrupted by mutant L173P. It is worth noting that the activating mutant L173F also showed enhanced correlated motions, which could be an explanation for the left shift curve of the ERK activity. The addition of L-Phe was unable to make the profile of mutant P221L into a more dramatically active state, which correlated well with the in vitro studies, suggesting the mutation is already in a “fully active” state upon the binding with Ca²⁺ similar as WT CaSR with both Ca²⁺ and L-Phe.

Principal component analysis (PCA) separates out the protein motions into principal modes ranked according to their relative contributions [27]. The analysis was done on the trajectories of the four mutations and the WT CaSR from the molecular dynamics simulations in order to predict the effect of the mutations on the response of L-Phe and Ca²⁺ at the atomic level (Figure 9).

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Figure 9. Principal component analysis (PCA) of the CaSR ECDs.

The trajectories of the molecular dynamics simulations were analyzed using principal component analysis (PCA), which separates out the motions of the CaSR ECD into principal modes ranked according to their relative contributions. The first three principal modes were included in the present study to analyze the four CaSR mutants and the wild type: WT (black), L173F (green), presence P221L (magenta), L173P (red), P221Q (blue).

https://doi.org/10.1371/journal.pone.0113622.g009

Projection of the trajectories of the CaSR mutants in both the apo forms (upper panel) and the holo forms (lower panel) onto the first three modes that accounted for the majority of the total fluctuations is shown in Figure 9. In the absence of Ca²⁺ and L-Phe, the conformations sampled by the CaSR mutants are similar to that of the WT CaSR. However, in the L-Phe and Ca²⁺-loaded forms, the conformations of the CaSR mutants L173P and L173F are distinguishable from the other mutants and WT CaSR (Figure 9). The results suggest that Ca²⁺ and L-Phe might shift the population of conformational ensembles of mutant L173P and L173F in a different way than in WT CaSR or in the other two mutants, supporting the essential role for the residue Leu¹⁷³ in the molecular connectivity underlying the Ca²⁺ - and Phe-mediated functional cooperativity of CaSR.

Loss-of-function mutations impair the functional cooperativity of the CaSR

The CaSR hinge region is considered to be crucial for the sensing of agonists (e.g., polyvalent cations and amino acids) [19], [28]. Mutations near Ca²⁺-binding site 1 are hypothesized to influence the functional cooperativity of the entire receptor. In vitro studies showed that the L173P and P221Q mutants produce a receptor with reduced positive homotropic cooperativity and impaired capability to sense [Ca²⁺]_o, while the L173F and P221L mutants enhance the sensitivity of the CaSR to [Ca²⁺]_o compared to the WT receptor, but barely change the cooperativity of the receptor. The results with the loss-of-function mutants indicate that Leu¹⁷³ and Pro²²¹ are important in maintaining the positive homotropic cooperativity among the receptor's calcium-binding sites. Conversely, the gain-of-function mutants show that these residues also are important for restraining the receptor from assuming its most active form (e.g., that seen in the WT CaSR in the presence of both high [Ca²⁺]_o and L-Phe). Thus unlike mutations of residues that cause only gain- or loss-of-function, Leu¹⁷³ and Pro²²¹ serve dual functions, perhaps reflecting their key locations in the cleft between the receptor's two lobes and their proximities to the binding sites for both [Ca²⁺]_o and L-Phe.

[Ca²⁺]_i oscillations have been postulated to be the result of complex responses from multiple signaling pathways. The [Ca²⁺]_o-triggered [Ca²⁺]_i oscillation pattern is believed to be modulated by the activity of phosphoinositide pathway [29] as well as a negative feedback loop involving the inhibitory effect of protein kinase C (PKC) on IP₃ production [30]. Downstream intracellular signaling responses, such as the production of IP₁ and the activity of ERK_1/2, which will be discussed later, can be reflected in the oscillation patterns associated with the different disease-related mutations. Although most research has focused on intracellular calcium mobilization in HEK293 cells heterologously expressing the CaSR, CaSR-induced [Ca²⁺]_i oscillations have also been found in cells with endogenously expressed CaSR, for instance in parathyroid cells [31], bovine anterior pituitary cells [32], opossum kidney (OK) cells [33] and medullary thyroid carcinoma cells [34]. Oscillations in [Ca²⁺]_i modulate not only the rate of parathyroid hormone (PTH) secretion, but also gene expression and other processes [35]–[38]. The pattern of [Ca²⁺]_i oscillations is thus one of the most important signatures reflecting the state of CaSR activity. In the present study, we showed that the inactive mutants required higher [Ca²⁺]_o to trigger intracellular [Ca²⁺]_i oscillations compared to the WT CaSR, while the active mutants needed lower [Ca²⁺]_o to initiate oscillations. The oscillation frequency of the inactive mutants remained similar to that of the WT CaSR, but higher [Ca²⁺]_i oscillations frequencies were observed in HEK293 cells transfected with gain-of-function mutations. As [Ca²⁺]_o is increased, the [Ca²⁺]_i oscillations cease and reach a plateau in CaSR-transfected cells as the desensitization process occurs and/or there is depletion of [Ca²⁺]_i stores. Although the loss-of-function mutation L173P still oscillated at 30.0 mM [Ca²⁺]_o, considering the elevated and broad range of its sensitivity to [Ca²⁺]_o, it is possible that a plateau would be reached at even higher levels of [Ca²⁺]_o.

Our studies of CaSR-mediated accumulation IP₁ (a metabolite of IP₃) and activation of the ERK_1/2 pathways in HEK293 cells transfected with the various CaSR mutants conveyed two messages: Firstly, the mutations caused changes in the patterns of [Ca²⁺]_i oscillations that at least partially involve activation of the Gα_q/11 pathway. The changes in the accumulation of IP₁ upon stimulation with various concentrations of [Ca²⁺]_o correlated well with [Ca²⁺]_i changes. Secondly, high [Ca²⁺]_o-evoked increases in ERK_1/2 activities of WT-CaSR and its four mutants were in accordance with the accumulation of IP₁. Stimulation of the ERK_1/2 activity in CaSR-transfected HEK293 cells has been reported to involve PKC-mediated as well as a PTX-sensitive, tyrosine kinase-dependent pathways [39]. Although it is believed that the carboxyl terminus of the protein is involved in the activation of MAPK signaling by interacting with the scaffold protein, filamin A [40], [41], other data showed that that alterations in the extracellular domain can also affect the ERK cascade, which is in agreement with previous studies [42]. Since all the mutations studied here were expressed at similar levels on the cell membrane (Figure 1b), these downstream signaling changes may at least partially be contributed by the disruption of cooperativity among different calcium binding sites introduced by different mutations. On the other hand, the gain-of-function mutations may increase the stability the receptor as suggested by other studies [43], or they possibly induce more correlated motions among the calcium-binding sites as suggested by the dynamic cross-correlation map.

L-Phe-induced heterotropic cooperativity rescues activity of the CaSR

In the current study, we showed that L-Phe could affect one or two of the three parameters depicting the [Ca²⁺]_o triggered [Ca²⁺]_i oscillation patterns in all of the mutants as measured in individual cellular responses (e.g., [Ca²⁺]_i oscillation starting points, ending points and [Ca²⁺]_i oscillation frequencies). The alterations in the oscillation patterns after the introduction of the allosteric activator could be the result of the activation of signaling downstream of G_α12/13 or a combined effect with the positive heterotropic cooperativity between the multiple Ca²⁺ binding sites induced by L-Phe. The interaction between CaSR and L-Phe has been reported to modulate the G_α12/13-RhoA pathway [44]; however, L-Phe could also potentially enhance [Ca²⁺]_o-induced alterations in intracellular signaling pathways through G_q/11 pathway as reflected by the IP₁ accumulation assay.

The present study also confirms that the ERK signaling pathway in CaSR-transfected HEK293 cells can be modulated not only by [Ca²⁺]_o but also by the positive allosteric modulator, L-Phe [45]. It should be noted that the ability of L-Phe to enhance the intracellular responses of the various signaling pathways varied between different mutations. L-Phe enhanced the maximum responses of the ERK signaling pathway to a greater extent than it altered the apparent affinities in the two gain-of-function mutants, although it barely showed any influence on the [Ca²⁺]_o-triggered [Ca²⁺]_i and IP₁ responses for these mutants, which may explain, in part, the dramatic changes in correlated motions from the computational simulation results using molecular dynamics. As a signaling messenger further downstream than either IP₁ or [Ca²⁺]_i, the phosphorylation of ERK_1/2 may not merely reflect activation of the G_q/11 pathway, but could be contributed to by other G-proteins (e.g., G_i). In agreement with the in silico results, L-Phe exhibited less effect on the regulation of the ERK_1/2 pathway in mutant L173P. The predicted L-Phe-binding site has been reported to be located near the hinge region between lobe 1 and lobe 2 and to involve residues S169A/S170A/S171A [46]. Leu173 is located within 5 Å of this region. The Leu to Pro change could potentially generate additional steric effects on the closure of lobe 1 and lobe 2 so that addition of exogenous L-Phe has relatively little impact on the CaSR's response to changes in [Ca²⁺]_o. Thus, the hetero-cooperativity between the allosteric modulator and calcium binding sites could be disturbed, resulting in the lack of a left-shifted concentration-response curve in response to [L-Phe].

Disease-associated mutations influence the molecular connectivity between the Ca²⁺-binding sites of the CaSR as revealed by molecular dynamic simulations

Based on all of the in vitro results and the in silico simulations, we propose a model to illustrate the possible mechanism by which the disease-associated mutations affect the function of CaSR and how their activities can be modulated by Ca²⁺ and L-Phe through the molecular connectivity that is encoded at the hinge region of the ECD of the protein. As shown in Fig 10, there are four functional states of the CaSR. In the absence of Ca²⁺, WT CaSR is at the basal state can be converted to the active state upon Ca²⁺ binding to site 1 at the hinge region. Subsequent binding of L-Phe at the adjacent hinge region further strengthens molecular connectivity and elevates the receptor to a “fully active” status in the presence of high [Ca²⁺]_o by a heterotropic positive cooperative mechanism. Mutations close to the hinge region such as those at Leu¹⁷³ and Pro²²¹ affect the overall dynamic correlated motions of the respective mutant receptors and the Ca²⁺-induced homotropic cooperativity, which could further influence the conformation of the receptor. The inactivating mutation L173P impairs the molecular connectivity in the WT CaSR and cannot be rescued by L-Phe. On the other hand, the inactivating mutation P221Q weakens the connectivity but can be partially rescued by binding Phe with a resultant activity similar to the basal activity of the WT CaSR. In contrast, the gain-of-function mutants L173F and P221L may already have been converted to the active state with dynamic properties similar to that of Ca²⁺- loaded WT CaSR. They can be further activated upon binding extracellular Ca²⁺ to reach a state close to the “fully active” state (Phe + [Ca²⁺]_o in the WT) that is insensitive to further activation by L-Phe achieving an apparent “ceiling” represented by the maximal achievable activity of the CaSR.

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Figure 10. Schematic representation of the mechanisms underlying the effects of the mutations on the CaSR and the modulation of receptor activity by extracellular Ca²⁺ and L-Phe.

Ca²⁺ and L-Phe modulate the activity as well as the cooperativity of CaSR (the color changes of the receptor from white to red indicates an increase in functional activity). Elevating [Ca²⁺]_o, e.g., to 3.0 mM, is proposed to change the basal CaSR status into an active form in a positive homotropic cooperative manner and further trigger [Ca²⁺]_i oscillations. L-Phe binds to the hinge region between lobe 1 and lobe 2, modulating the receptor together with Ca²⁺ in a positive heterotropic cooperative way. This could potentiate conversion of the receptor to a “fully active” form associated with a higher frequency of [Ca²⁺]_i oscillations and a left-shifted EC₅₀. Loss-of-function CaSR mutants (indicated by white color) could cause a disruption of the cooperativity among the various Ca²⁺-binding sites (dashed arrows). [Ca²⁺]_o at 3.0 mM does not trigger [Ca²⁺]_i oscillations in the mutant CaSRs. The impaired receptor function and the cross-talk between Ca²⁺-binding sites can be at least partially rescued for some mutants by L-Phe (e.g.P221Q). However, if the mutation interferes the interaction between CaSR and L-Phe, the function of the receptor may not be fully recovered (e.g.L173P). CaSR gain-of-function mutants (left) exhibit enhanced correlated motions (double line arrows) and their activity is not further potentiated by L-Phe, potentially due to a ceiling effect.

https://doi.org/10.1371/journal.pone.0113622.g010

The results from PCA may also provide an explanation as to why L-Phe interacts preferably with the P221Q mutant. L-Phe could not effectively shift the inactive population of conformational ensembles of L173P to an active ensemble of conformations as it did with the other inactivating mutant and WT. However, whether the in silico simulations provide a novel method to predict mutational effects on CaSR will ultimately require the kind of structural detail available for the mGluRs, whose structures have been solved. Here, the model structure does not take into consideration the possible interactions between the ECD and the extracellular loops and/or transmembrane domain as well as the intracellular domains. Thus, caution is merited in over-interpreting the in silico results. Nevertheless, the predicted structural motions may help to identify the mutations or ligands that produce increased or diminished coupling among specific structural elements within the CaSR ECD. While this type of analysis is limited at the moment to the environment of the orthosteric site, this strategy could facilitate drug design for modulating the functions of disease-related mutations affecting the CaSR, as well as other members of the family C GPCRs.

The distinctive combinatorial effects of L-Phe and [Ca²⁺]_o on different mutant CaSRs also suggests that ligand selectivity at the orthosteric site and receptor activity at non-orthosteric sites could both contribute to the function of the receptor. This feature of the adjacent orthosteric binding sites and allosteric regulatory sites that cooperatively regulate the molecular connectivity may be shared by other members of the family C GPCRs [47], [48]. In addition to the Ca²⁺-sensing receptor, [Ca²⁺]_o regulates 14 of the other members of the family C G protein-coupled receptors (GPCRs), including the metabotropic glutamate receptors (mGluR), γ-aminobutyric acid GABA_B receptors and receptors for pheromones, amino acids and sweet substances [1], [49]–[55]. The observed molecular modulatory mechanism may be shared by other members of the family C GPCRs [47], [48]. For instance, the analyses of multiple point mutations in the taste receptors (T1R1/T1R3) suggested that the combination of the two distinct determinants selectivity at the ligand binding site and receptor activity at allosteric sites may mediate the ligand specificity of T1R1/T1R3 [56].

In conclusion, through analysis of [Ca²⁺]_i oscillations and the [Ca²⁺]_o- and L-Phe triggered downstream signaling changes, functional positive cooperativity of CaSR was revealed to be disrupted in the identified FHH-associated CaSR mutations, L173P and P221Q, but not in the ADHH related mutations, L173F and P221L. The addition of L-Phe rescued the function of P221Q by inducing heterotropic positive cooperativity. The distinctive correlated motions of the gain-of-function mutations, on the one hand, and the loss-of-function mutations, on the other, and the potential utility revealed by the molecular dynamic simulation in providing insights into the mechanism for the disease-associated functional alterations in the receptor may also apply to other GPCR proteins.