Citrate Synthase Expression Affects Tumor Phenotype and Drug Resistance in Human Ovarian Carcinoma
Figure 6
Gene expression profile related to drug resistance and apoptosis in SKOV3 and A2780 cells after CS silencing.
(A, B) Ovarian cancer cells were treated with siRNA for 48 h, total RNA was extracted for gene expression analysis. Expression of CASP7 (CASPASE7), IRF7 (interferon regulatory factor 7), DDX58 (DEAD (Asp-Glu-Ala-Asp) box polypetide 58), and ISG15 (IFN-stimulated gene 15) was increased significantly whereas ATG12 (autophagy related 12) expression was decreased in SKOV3 and A2780 cells after CS knockdown. β-actin was used as an internal control gene. Mean ± SEM. *P<0.05, **P<0.01 and ***P<0.001. (C) The diagram was shown to identify potential signaling pathways modulated by CS.