Heatmap represents a two-way clustering analysis derived from lipid profiles. Vertical dendrogram represents clustering of profiles by components. This dendrogram shows components clustering together at various points in the tree. The dot plot immediately to the right of the vertical diagram represents each of the eleven hives sampled and indicates that communities are not clustering by hive as dots are not found in groups on the same row. Each of the sample labels are color coded to represent sampling year. Similar to component-wise consideration, there appear to be clusters by year, although to a lesser extent. The horizontal dendrogram at the top of the figure represents individual lipids. Clustering can also be observed when considering general lipid division, e.g. the monounsaturated fatty acids group together. Fatty acid coloring: hydroxyl, green; cyclic, orange; branched, purple; monounsaturated, red; saturated, blue; methylated, yellow; polyunsaturated, pink; unclassified, black.

https://doi.org/10.1371/journal.pone.0133100.g001