Figures
In Fig 1, the orientation of vector-specific 5’ and 3’ sequences for pEpic and pEpic_Lite and derivatives of these vectors are incorrect. The correct configurations are all sense-strand oriented components. Please see the corrected Fig 1 here.
(A) Schematic of an LR recombination reaction and the resulting vector. Site-specific recombination events (red lines) between attR and attL sites from a 5’, middle, and 3’ entry vector with a destination vector replaces the ccdB/CmR selection cassette of the destination vector with the mobile DNA elements from the entry vectors, leaving destination vector-specific 5’ and 3’ sequences intact. (B) Schematic of lentiviral destination vectors pEpic and pEpic_Lite. attR3 and 4 sites flanking the ccdB/CmR selection cassette are positioned in an anti-sense orientation to viral RNA expression driven by a Rous sarcoma virus (RSV) promoter. pEpic_Lite lacks puromycin resistance (PuroR). LTR = long terminal repeat; RRE = Rev response element; cPPT = central polypurine tract; ccdB = E. coli ccdB toxin; CmR = chloramphenicol resistance; mPGK = mouse phosphoglycerate kinase promoter; WPRE = woodchuck hepatitis virus posttranslational regulatory element.
The correct plasmids and their sequences have been deposited with Addgene (www.addgene.org), with the following catalog numbers: pEpic #84372; pEpic_Lite #84373.
Reference
Citation: Fowler DK, Stewart S, Seredick S, Eisen JS, Stankunas K, Washbourne P (2017) Correction: A MultiSite Gateway Toolkit for Rapid Cloning of Vertebrate Expression Constructs with Diverse Research Applications. PLoS ONE 12(4): e0176543. https://doi.org/10.1371/journal.pone.0176543
Published: April 20, 2017
Copyright: © 2017 Fowler et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.